On-chip fabrication of mutifunctional envelope-type nanodevices for gene delivery

Hiroshi Kuramoto, Yeon Su Park, Noritada Kaji, Manabu Tokeshi, Kentaro Kogure, Yasuo Shinohara, Hideyoshi Harashima, Yoshinobu Baba

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

Microfluidic devices may be highly beneficial to the rapid fabrication of small quantities of various nonviral vectors with different functionalities, which is indispensable for effective order-made gene therapy. We adapted a microfluidic chip-based approach for fabricating small quantities of nonviral vectors in a short time in preparation for order-made gene therapy applications. This approach permitted us to fabricate multifunctional envelope-type nanodevices (MENDs), composed of a compacted (or condensed) DNA core and a lipid bilayer membrane shell, which are considered as promising nonviral vectors for gene therapy applications. The on-chip fabrication of the MEND was very simple, rapid, convenient, and cost-effective compared with conventional methods. The size of the MEND showed strong dependence on the concentration and flow rate of the reaction precursors and could be controlled to be much smaller than that achievable by conventional methods. This, together with abovementioned merits, makes our microfluidic chip-based approach very attractive for the fabrication of MENDs for effective application to order-made gene therapy.

本文言語英語
ページ(範囲)2729-2733
ページ数5
ジャーナルAnalytical and Bioanalytical Chemistry
391
8
DOI
出版ステータス出版済み - 8 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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