On-chip temperature sensing and control for cell immobilization and culture system

Yoko Yamanishi, Edward Chow, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

1 被引用数 (Scopus)

抄録

This paper presents a temperature sensing and controlling microfluid chip for cell immobilization using a thermo-sensitive hydrogel (PNIPAAm). The ITO (Indium Tin Oxide) microheaters fabricated by micromachining technology, perform heating of the solution of cell, PNIPAAm and Calcein-AM (fluorescent dye) in microchannels. It is important to note that a thick gel formation cause to block the observation of culturing cells due to the autofluorescence of gel which reduce the S/N ratio. For the present study, we are targeting to fabricate a suspended micro-bridge above a microheater to limit the height of gel to form "thin and transparent gel layer" above the heater. The research was focused on controlling of gel thickness which eliminated unnecessary interferences of autofluorescence of gel in the observation by the inverted microscope, and a better S/N ratio can be obtained than the conventional observation with uncontrolled gel thickness. All the heating and a suspended biocompatible micro-bridge were integrated on a chip, in which yeast cells immobilization can be performed by the gelation of the PNIPAAm solution.

本文言語英語
ホスト出版物のタイトル2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
ページ295-300
ページ数6
DOI
出版ステータス出版済み - 2008
外部発表はい
イベント2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, 日本
継続期間: 11 6 200811 9 2008

出版物シリーズ

名前2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

その他

その他2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
国/地域日本
CityNagoya
Period11/6/0811/9/08

All Science Journal Classification (ASJC) codes

  • コンピュータ ビジョンおよびパターン認識
  • 人間とコンピュータの相互作用
  • 電子工学および電気工学

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