On-line sample preconcentration by large-volume sample stacking with an electroosmotic flow pump (LVSEP) in microscale electrophoresis

Fumihiko Kitagawa, Takayuki Kawai, Koji Otsuka

研究成果: Contribution to journalReview article査読

24 被引用数 (Scopus)

抄録

This review highlights on-line sample preconcentration by large-volume sample stacking with an electroosmotic flow pump (LVSEP) in microscale electrophoresis. LVSEP requires only a hydrodynamic sample injection into the whole capillary/microchannel and a constant voltage application, i.e., without polarity switching, providing an efficient sensitivity enhancement with a relatively easy experimental procedure without loss of the separation efficiencies. Recent developments and applications of LVSEP are briefly reviewed from the first LVSEP study in 1999 with categorization on the basis of separation modes, including zone electrophoresis, cyclodextrin- and affinity-based separation modes and non-aqueous capillary electrophoresis.

本文言語英語
ページ(範囲)1129-1139
ページ数11
ジャーナルanalytical sciences
29
12
DOI
出版ステータス出版済み - 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学

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