On the mechanism of cell lysis by deformation

Hiroshi Takamatsu, Ryu Takeya, Seiji Naito, Hideki Sumimoto

研究成果: Contribution to journalArticle査読

31 被引用数 (Scopus)

抄録

In this study, we identify the extent of deformation that causes cell lysis using a simple technique where a drop of cell suspension is compressed by two flat plates. The viability of human prostatic adenocarcinoma PC-3 cells in solutions of various concentrations of NaCl is determined as a function of the gap size between the plates. The viability declines with decreasing gap size in the following order: 700mM > 150mM > 75mM NaCl. This is considered to be due to the difference in cell size, which is caused by the osmotic volume change before deformation; cell diameter becomes smaller in a solution of higher NaCl concentration, which appears to increase the survival ratio in a given gap size. The deformation-induced decrease in cell viability is correlated with the cell surface strain, which is dependent on the increase in surface area, irrespective of NaCl concentration. In addition, the treatment of cells with cytochalasin D results in the disappearance of cortical actin filaments and a marked drop in the viability, indicating that cell lysis is closely related to the deformation of the cytoskeleton.

本文言語英語
ページ(範囲)117-124
ページ数8
ジャーナルJournal of Biomechanics
38
1
DOI
出版ステータス出版済み - 1 2005

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 整形外科およびスポーツ医学
  • 生体医工学
  • リハビリテーション

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