One-end immobilization of individual dna molecules on a functional hydrophobic glass surface

Shun Ichi Matsuura, Hirofumi Kurita, Michihiko Nakano, Jun Komatsu, Kazunori Takashima, Shinji Katsura, Akira Mizuno

研究成果: ジャーナルへの寄稿学術誌査読

13 被引用数 (Scopus)

抄録

We demonstrate an effective method for DNA immobilization on a hydrophobic glass surface. The new DNA immobilizing technique is extremely simple compared with conventional techniques that require heterobifunctional crosslinking reagent between DNA and substrate surface that are both modified chemically. In the first process, a coverslip was treated with dichlorodimethylsilane resulting in hydrophobic surface. γ DNA molecules were ligated with 3′-terminus disulfide-modified 14 mer oligonucleotides at one cohesive end. After reduction of the disulfide to sulfhydryl (thiol) groups the resulting thiol-modified γ DNA molecules were reacted on silanized coverslip. Fluorescent observation showed that the thiol-modified γ DNA molecules were anchored specifically to the hydrophobic surface at one terminus, although non-specific binding of the DNA molecules was suppressed. It was observed that the one-end-attached DNA molecule was bound firmly to the surface and stretched reversibly in one direction when a d.c. electric field was applied.

本文言語英語
ページ(範囲)429-436
ページ数8
ジャーナルJournal of Biomolecular Structure and Dynamics
20
3
DOI
出版ステータス出版済み - 12月 2002
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 分子生物学

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