One-step synthesis of cellooligomer-conjugated gold nanoparticles in a water-in-oil emulsion system and their application in biological sensing

Mayumi Hatakeyama, Daisuke Ryuno, Shingo Yokota, Hirofumi Ichinose, Takuya Kitaoka

研究成果: ジャーナルへの寄稿記事

抄録

Monodisperse gold nanoparticles (GNPs) were synthesized in a water-in-oil emulsion system (reverse micelles) composed of 80% N-methylmorpholine N-oxide (NMMO)/20% H 2 O and dodecane, stabilized with an anionic surfactant: bis(2-ethylhexyl)sulfosuccinate sodium salt. Cellooligomers with a degree of polymerization of 6 or 15 (βGlc6 or βGlc15, respectively), which were labeled at each reducing end group with thiosemicarbazide (TSC) and dissolved in the aqueous NMMO phase, were successfully conjugated to the surfaces of GNPs in situ during spontaneous NMMO-mediated gold reduction. As-synthesized βGlc6-GNPs and βGlc15-GNPs had average diameters of 11.3 ± 2.1 and 10.5 ± 0.7 nm, respectively, while their surface sugar densities were 0.21 and 0.51 chains nm –2 , respectively. Concanavalin A (ConA), a lectin that recognizes non-reducing end groups of glucose residues, aggregated with βGlc15-GNPs with higher sensitivity than it did with βGlc6-GNPs, possibly as a result of the sugar density on the GNP surfaces. The aggregates were rapidly re-suspended by adding methyl-β-D-glucopyranoside as a binding inhibitor. Other lectins and proteins showed no interaction with βGlc-GNPs. Therefore, clustering of glucose non-reducing ends on the GNP surfaces via strong intermolecular association of cellooligomers, possibly led to high affinity for ConA. This facile synthesis route to structural carbohydrate-decorated GNPs has potential applications in carbohydrate–nanometal conjugate nano-biosensor development.

元の言語英語
ページ(範囲)74-79
ページ数6
ジャーナルColloids and Surfaces B: Biointerfaces
178
DOI
出版物ステータス出版済み - 6 1 2019

Fingerprint

Emulsions
Gold
Nanoparticles
emulsions
Oils
oils
gold
nanoparticles
Water
synthesis
water
sugars
Concanavalin A
Lectins
glucose
Sugars
Oxides
Glucose
oxides
Dioctyl Sulfosuccinic Acid

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

これを引用

@article{84c2950b21c144f685d212ca50f33ba2,
title = "One-step synthesis of cellooligomer-conjugated gold nanoparticles in a water-in-oil emulsion system and their application in biological sensing",
abstract = "Monodisperse gold nanoparticles (GNPs) were synthesized in a water-in-oil emulsion system (reverse micelles) composed of 80{\%} N-methylmorpholine N-oxide (NMMO)/20{\%} H 2 O and dodecane, stabilized with an anionic surfactant: bis(2-ethylhexyl)sulfosuccinate sodium salt. Cellooligomers with a degree of polymerization of 6 or 15 (βGlc6 or βGlc15, respectively), which were labeled at each reducing end group with thiosemicarbazide (TSC) and dissolved in the aqueous NMMO phase, were successfully conjugated to the surfaces of GNPs in situ during spontaneous NMMO-mediated gold reduction. As-synthesized βGlc6-GNPs and βGlc15-GNPs had average diameters of 11.3 ± 2.1 and 10.5 ± 0.7 nm, respectively, while their surface sugar densities were 0.21 and 0.51 chains nm –2 , respectively. Concanavalin A (ConA), a lectin that recognizes non-reducing end groups of glucose residues, aggregated with βGlc15-GNPs with higher sensitivity than it did with βGlc6-GNPs, possibly as a result of the sugar density on the GNP surfaces. The aggregates were rapidly re-suspended by adding methyl-β-D-glucopyranoside as a binding inhibitor. Other lectins and proteins showed no interaction with βGlc-GNPs. Therefore, clustering of glucose non-reducing ends on the GNP surfaces via strong intermolecular association of cellooligomers, possibly led to high affinity for ConA. This facile synthesis route to structural carbohydrate-decorated GNPs has potential applications in carbohydrate–nanometal conjugate nano-biosensor development.",
author = "Mayumi Hatakeyama and Daisuke Ryuno and Shingo Yokota and Hirofumi Ichinose and Takuya Kitaoka",
year = "2019",
month = "6",
day = "1",
doi = "10.1016/j.colsurfb.2019.02.051",
language = "English",
volume = "178",
pages = "74--79",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

TY - JOUR

T1 - One-step synthesis of cellooligomer-conjugated gold nanoparticles in a water-in-oil emulsion system and their application in biological sensing

AU - Hatakeyama, Mayumi

AU - Ryuno, Daisuke

AU - Yokota, Shingo

AU - Ichinose, Hirofumi

AU - Kitaoka, Takuya

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Monodisperse gold nanoparticles (GNPs) were synthesized in a water-in-oil emulsion system (reverse micelles) composed of 80% N-methylmorpholine N-oxide (NMMO)/20% H 2 O and dodecane, stabilized with an anionic surfactant: bis(2-ethylhexyl)sulfosuccinate sodium salt. Cellooligomers with a degree of polymerization of 6 or 15 (βGlc6 or βGlc15, respectively), which were labeled at each reducing end group with thiosemicarbazide (TSC) and dissolved in the aqueous NMMO phase, were successfully conjugated to the surfaces of GNPs in situ during spontaneous NMMO-mediated gold reduction. As-synthesized βGlc6-GNPs and βGlc15-GNPs had average diameters of 11.3 ± 2.1 and 10.5 ± 0.7 nm, respectively, while their surface sugar densities were 0.21 and 0.51 chains nm –2 , respectively. Concanavalin A (ConA), a lectin that recognizes non-reducing end groups of glucose residues, aggregated with βGlc15-GNPs with higher sensitivity than it did with βGlc6-GNPs, possibly as a result of the sugar density on the GNP surfaces. The aggregates were rapidly re-suspended by adding methyl-β-D-glucopyranoside as a binding inhibitor. Other lectins and proteins showed no interaction with βGlc-GNPs. Therefore, clustering of glucose non-reducing ends on the GNP surfaces via strong intermolecular association of cellooligomers, possibly led to high affinity for ConA. This facile synthesis route to structural carbohydrate-decorated GNPs has potential applications in carbohydrate–nanometal conjugate nano-biosensor development.

AB - Monodisperse gold nanoparticles (GNPs) were synthesized in a water-in-oil emulsion system (reverse micelles) composed of 80% N-methylmorpholine N-oxide (NMMO)/20% H 2 O and dodecane, stabilized with an anionic surfactant: bis(2-ethylhexyl)sulfosuccinate sodium salt. Cellooligomers with a degree of polymerization of 6 or 15 (βGlc6 or βGlc15, respectively), which were labeled at each reducing end group with thiosemicarbazide (TSC) and dissolved in the aqueous NMMO phase, were successfully conjugated to the surfaces of GNPs in situ during spontaneous NMMO-mediated gold reduction. As-synthesized βGlc6-GNPs and βGlc15-GNPs had average diameters of 11.3 ± 2.1 and 10.5 ± 0.7 nm, respectively, while their surface sugar densities were 0.21 and 0.51 chains nm –2 , respectively. Concanavalin A (ConA), a lectin that recognizes non-reducing end groups of glucose residues, aggregated with βGlc15-GNPs with higher sensitivity than it did with βGlc6-GNPs, possibly as a result of the sugar density on the GNP surfaces. The aggregates were rapidly re-suspended by adding methyl-β-D-glucopyranoside as a binding inhibitor. Other lectins and proteins showed no interaction with βGlc-GNPs. Therefore, clustering of glucose non-reducing ends on the GNP surfaces via strong intermolecular association of cellooligomers, possibly led to high affinity for ConA. This facile synthesis route to structural carbohydrate-decorated GNPs has potential applications in carbohydrate–nanometal conjugate nano-biosensor development.

UR - http://www.scopus.com/inward/record.url?scp=85062294353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062294353&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2019.02.051

DO - 10.1016/j.colsurfb.2019.02.051

M3 - Article

VL - 178

SP - 74

EP - 79

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -