TY - JOUR
T1 - Optical characterization of the antigen-antibody thin layer using the whispering gallery mode
AU - Tajiri, Takeshi
AU - Matsumoto, Shuzo
AU - Imato, Toshihiko
AU - Okamoto, Toshihiro
AU - Haraguchi, Masanobu
N1 - Publisher Copyright:
© The Japan Society for Analytical Chemistry.
PY - 2014
Y1 - 2014
N2 - We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e., thickness and refractive index, of anti-β-Galactosidase on the sphere surface were evaluated based on an absorbance measurement and a resonance peak shift measurement, respectively. Moreover, we measured the variation of the WGM spectra depending on the concentration of the enzyme solution (β-Galactosidase), which allowed us to optically evaluate the thickness and the refractive index of the antigen-antibody layer from the shift of the WGM spectra peak. 2014
AB - We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e., thickness and refractive index, of anti-β-Galactosidase on the sphere surface were evaluated based on an absorbance measurement and a resonance peak shift measurement, respectively. Moreover, we measured the variation of the WGM spectra depending on the concentration of the enzyme solution (β-Galactosidase), which allowed us to optically evaluate the thickness and the refractive index of the antigen-antibody layer from the shift of the WGM spectra peak. 2014
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U2 - 10.2116/analsci.30.799
DO - 10.2116/analsci.30.799
M3 - Article
C2 - 25109641
AN - SCOPUS:84924197337
SN - 0910-6340
VL - 30
SP - 799
EP - 804
JO - Analytical Sciences
JF - Analytical Sciences
IS - 8
ER -