Optical clearing of fixed brain samples using SeeDB

Meng Tsen Ke, Takeshi Imai

研究成果: ジャーナルへの寄稿記事

29 引用 (Scopus)

抄録

Large-scale three-dimensional fluorescence imaging is essential for comprehensive and quantitative understanding of neuronal circuitry.We describe a water-based optical clearing agent, SeeDB, which clears fixed brain samples in a few days leaving many types of fluorescent dyes unquenched, including fluorescent proteins and lipophilic neuronal tracers. This method maintains a constant sample volume during the clearing procedure, an important factor to keep cellular morphology intact. After optical clearing with SeeDB, we can reach a depth of >1000 μm with confocal microscopy. When combined with two-photon microscopy, SeeDB allows us to image fixed mouse brains at millimeter-scale level.

元の言語英語
記事番号2.22
ジャーナルCurrent Protocols in Neuroscience
発行部数SUPPL.66
DOI
出版物ステータス出版済み - 1 1 2014

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Three-Dimensional Imaging
Optical Imaging
Brain
Fluorescent Dyes
Photons
Confocal Microscopy
Microscopy
Water
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

これを引用

Optical clearing of fixed brain samples using SeeDB. / Ke, Meng Tsen; Imai, Takeshi.

:: Current Protocols in Neuroscience, 番号 SUPPL.66, 2.22, 01.01.2014.

研究成果: ジャーナルへの寄稿記事

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