抄録
Large-scale three-dimensional fluorescence imaging is essential for comprehensive and quantitative understanding of neuronal circuitry.We describe a water-based optical clearing agent, SeeDB, which clears fixed brain samples in a few days leaving many types of fluorescent dyes unquenched, including fluorescent proteins and lipophilic neuronal tracers. This method maintains a constant sample volume during the clearing procedure, an important factor to keep cellular morphology intact. After optical clearing with SeeDB, we can reach a depth of >1000 μm with confocal microscopy. When combined with two-photon microscopy, SeeDB allows us to image fixed mouse brains at millimeter-scale level.
| 元の言語 | 英語 |
|---|---|
| 記事番号 | 2.22 |
| ジャーナル | Current Protocols in Neuroscience |
| 発行部数 | SUPPL.66 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2014 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
これを引用
Optical clearing of fixed brain samples using SeeDB. / Ke, Meng Tsen; Imai, Takeshi.
：: Current Protocols in Neuroscience, 番号 SUPPL.66, 2.22, 01.01.2014.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Optical clearing of fixed brain samples using SeeDB
AU - Ke, Meng Tsen
AU - Imai, Takeshi
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Large-scale three-dimensional fluorescence imaging is essential for comprehensive and quantitative understanding of neuronal circuitry.We describe a water-based optical clearing agent, SeeDB, which clears fixed brain samples in a few days leaving many types of fluorescent dyes unquenched, including fluorescent proteins and lipophilic neuronal tracers. This method maintains a constant sample volume during the clearing procedure, an important factor to keep cellular morphology intact. After optical clearing with SeeDB, we can reach a depth of >1000 μm with confocal microscopy. When combined with two-photon microscopy, SeeDB allows us to image fixed mouse brains at millimeter-scale level.
AB - Large-scale three-dimensional fluorescence imaging is essential for comprehensive and quantitative understanding of neuronal circuitry.We describe a water-based optical clearing agent, SeeDB, which clears fixed brain samples in a few days leaving many types of fluorescent dyes unquenched, including fluorescent proteins and lipophilic neuronal tracers. This method maintains a constant sample volume during the clearing procedure, an important factor to keep cellular morphology intact. After optical clearing with SeeDB, we can reach a depth of >1000 μm with confocal microscopy. When combined with two-photon microscopy, SeeDB allows us to image fixed mouse brains at millimeter-scale level.
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UR - http://www.scopus.com/inward/citedby.url?scp=84905694063&partnerID=8YFLogxK
U2 - 10.1002/0471142301.ns0222s66
DO - 10.1002/0471142301.ns0222s66
M3 - Article
C2 - 24510778
AN - SCOPUS:84905694063
JO - Current Protocols in Neuroscience
JF - Current Protocols in Neuroscience
SN - 1934-8584
IS - SUPPL.66
M1 - 2.22
ER -