Optical tweezers with fluorescence detection for temperature-dependent microrheological measurements

Atsuomi Shundo, Koichiro Hori, David P. Penaloza, Keiji Tanaka

    研究成果: Contribution to journalArticle査読

    11 被引用数 (Scopus)

    抄録

    We introduce a setup of optical tweezers, capable of carrying out temperature-dependent rheological measurements of soft materials. In our setup, the particle displacement is detected by imaging a bright spot due to fluorescence emitted from a dye-labeled particle against a dark background onto a quadrant photodiode. This setup has a relatively wide space around the sample that allows us to further accessorize the optical tweezers by a temperature control unit. The applicability of the setup was examined on the basis of the rheological measurements using a typical viscoelastic system, namely a worm-like micelle solution. The temperature and frequency dependences of the local viscoelastic functions of the worm-like micelle solution obtained by this setup were in good accordance with those obtained by a conventional oscillatory rheometer, confirming the capability of the optical tweezers as a tool for the local rheological measurements of soft materials. Since the optical tweezers measurements only require a tiny amount of sample (∼40 μL), the rheological measurements using our setup should be useful for soft materials of which the available amount is limited.

    本文言語英語
    論文番号014103
    ジャーナルReview of Scientific Instruments
    84
    1
    DOI
    出版ステータス出版済み - 1 2013

    All Science Journal Classification (ASJC) codes

    • Instrumentation

    フィンガープリント 「Optical tweezers with fluorescence detection for temperature-dependent microrheological measurements」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル