固相免疫測定を目的とした融合タンパク質試薬の設計とヒト培養細胞による生産プロセスの最適化

林 浩之輔, 友添 祐介, 永井 賢治, 松葉 恭一, 三ツ森 正之, 平石 佳之, 松村 外志張, 上田 宏, 神谷 典穂

研究成果: Contribution to journalArticle査読

抄録

New fusion proteins for immunoassay were developed as an alternative to the conventional chemical linked enzyme-antibody complex. Human chimeric alkaline phosphatase (IPP) was used as the labeling enzyme, and partial domains of <i>Staphylococcus aureus</i> Protein A and <i>Streptococcus</i> Protein G were used as antibody binding protein (PG). The fusion protein composed of IPP and PG was produced using human cell lines because IPP is derived from human enzymes. The expression system was optimized by changing the plasmid vector, reducing the GC content of the DNA sequence, and employing different cell lines including adherent and suspension cells. As result, a 2,600-fold increase in the protein yield per unit of growth medium was achieved. The resultant IPP-PG fusion protein was successfully utilized for immunoassay applications such as western blotting and immunohistochemistry.
寄稿の翻訳タイトルOptimization of a Fusion Protein Expression System using Human Cell Lines to Create a Practical Immunoassay Reagent
本文言語日本語
ページ(範囲)38-42
ページ数5
ジャーナルKagaku Kogaku Ronbunshu
41
1
DOI
出版ステータス出版済み - 2015

フィンガープリント

「固相免疫測定を目的とした融合タンパク質試薬の設計とヒト培養細胞による生産プロセスの最適化」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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