Optimization of a pH-shift control strategy for producing monoclonal antibodies in Chinese hamster ovary cell cultures using a pH-dependent dynamic model

Tomoharu Hogiri, Hiroshi Tamashima, Akitoshi Nishizawa, Masahiro Okamoto

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

To optimize monoclonal antibody (mAb) production in Chinese hamster ovary cell cultures, culture pH should be temporally controlled with high resolution. In this study, we propose a new pH-dependent dynamic model represented by simultaneous differential equations including a minimum of six system component, depending on pH value. All kinetic parameters in the dynamic model were estimated using an evolutionary numerical optimization (real-coded genetic algorithm) method based on experimental time-course data obtained at different pH values ranging from 6.6 to 7.2. We determined an optimal pH-shift schedule theoretically. We validated this optimal pH-shift schedule experimentally and mAb production increased by approximately 40% with this schedule. Throughout this study, it was suggested that the culture pH-shift optimization strategy using a pH-dependent dynamic model is suitable to optimize any pH-shift schedule for CHO cell lines used in mAb production projects.

元の言語英語
ページ(範囲)245-250
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
125
発行部数2
DOI
出版物ステータス出版済み - 2 1 2018

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Monoclonal antibodies
Cricetulus
Cell culture
Ovary
Dynamic models
Cell Culture Techniques
Monoclonal Antibodies
Appointments and Schedules
Kinetic parameters
Antibody Formation
Differential equations
Genetic algorithms
CHO Cells
Cell Line

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Optimization of a pH-shift control strategy for producing monoclonal antibodies in Chinese hamster ovary cell cultures using a pH-dependent dynamic model. / Hogiri, Tomoharu; Tamashima, Hiroshi; Nishizawa, Akitoshi; Okamoto, Masahiro.

:: Journal of Bioscience and Bioengineering, 巻 125, 番号 2, 01.02.2018, p. 245-250.

研究成果: ジャーナルへの寄稿記事

Hogiri, Tomoharu ; Tamashima, Hiroshi ; Nishizawa, Akitoshi ; Okamoto, Masahiro. / Optimization of a pH-shift control strategy for producing monoclonal antibodies in Chinese hamster ovary cell cultures using a pH-dependent dynamic model. :: Journal of Bioscience and Bioengineering. 2018 ; 巻 125, 番号 2. pp. 245-250.
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