TY - JOUR
T1 - Optimization of extraction method for GC-MS based metabolomics for filamentous fungi
AU - Madla, Siribhorn
AU - Miura, Daisuke
AU - Wariishi, Hiroyuki
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - Metabolite extraction is considered as one of the most important for quality controlling steps in metabolomic research. In the present study, optimal extraction protocol for fungal intracellular metabolites was investigated. Two extraction protocols with different solvent systems, methanol extraction and biphasic extraction under different pH conditions were compared. Number of detected peaks, sample-to-sample variation, and throughput of whole process were taken into account as criteria. Extracted metabolites were analyzed by gas chromatography-mass spectrometry (GC-MS) and principal component analysis (PCA). The extraction protocol using methanol as extraction solvent provided the highest number of detected peaks (~ 300 peaks) with the ease and speed, suggesting that the methanol-extraction method was suitable for intracellular metabolites extraction from P. chrysosporium. However, depending on the nature of metabolites of interest, the biphasic extraction method under different pH conditions is suitable for a targeted analysis.
AB - Metabolite extraction is considered as one of the most important for quality controlling steps in metabolomic research. In the present study, optimal extraction protocol for fungal intracellular metabolites was investigated. Two extraction protocols with different solvent systems, methanol extraction and biphasic extraction under different pH conditions were compared. Number of detected peaks, sample-to-sample variation, and throughput of whole process were taken into account as criteria. Extracted metabolites were analyzed by gas chromatography-mass spectrometry (GC-MS) and principal component analysis (PCA). The extraction protocol using methanol as extraction solvent provided the highest number of detected peaks (~ 300 peaks) with the ease and speed, suggesting that the methanol-extraction method was suitable for intracellular metabolites extraction from P. chrysosporium. However, depending on the nature of metabolites of interest, the biphasic extraction method under different pH conditions is suitable for a targeted analysis.
UR - http://www.scopus.com/inward/record.url?scp=84859882897&partnerID=8YFLogxK
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U2 - 10.4172/1948-5948.1000064
DO - 10.4172/1948-5948.1000064
M3 - Article
AN - SCOPUS:84859882897
VL - 4
SP - 5
EP - 9
JO - Journal of Microbial and Biochemical Technology
JF - Journal of Microbial and Biochemical Technology
SN - 1948-5948
IS - 1
ER -