Optimization of isopropanol production by engineered cyanobacteria with a synthetic metabolic pathway

Yasutaka Hirokawa, Iwane Suzuki, Taizo Hanai

研究成果: Contribution to journalArticle査読

35 被引用数 (Scopus)

抄録

Cyanobacterium is an attractive host for the production of various chemicals and alternative fuels using solar energy and carbon dioxide. In previous study, we succeeded to produce isopropanol using engineered Synechococcus elongatus PCC 7942 under dark and anaerobic conditions (0.43mM, 26.5mg/l). In the present study, we report the further optimization of this isopropanol producing condition. We then optimized growth conditions for production of isopropanol by the engineered cyanobacteria, including the use of cells in early stationary phase and buffering of the production medium to neutral pH. We observed that shifting of cultures from dark and anaerobic to light and aerobic conditions during the production phase dramatically increased isopropanol production by conversion to isopropanol from acetate, byproduct under dark and anaerobic condition. Under the optimized production conditions, the titer of isopropanol was elevated 6-fold, to 2.42mM (146mg/l).

本文言語英語
ページ(範囲)585-590
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
119
5
DOI
出版ステータス出版済み - 5 1 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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