Optimization of isopropanol production by engineered cyanobacteria with a synthetic metabolic pathway

Yasutaka Hirokawa, Iwane Suzuki, Taizo Hanai

研究成果: ジャーナルへの寄稿記事

28 引用 (Scopus)

抄録

Cyanobacterium is an attractive host for the production of various chemicals and alternative fuels using solar energy and carbon dioxide. In previous study, we succeeded to produce isopropanol using engineered Synechococcus elongatus PCC 7942 under dark and anaerobic conditions (0.43mM, 26.5mg/l). In the present study, we report the further optimization of this isopropanol producing condition. We then optimized growth conditions for production of isopropanol by the engineered cyanobacteria, including the use of cells in early stationary phase and buffering of the production medium to neutral pH. We observed that shifting of cultures from dark and anaerobic to light and aerobic conditions during the production phase dramatically increased isopropanol production by conversion to isopropanol from acetate, byproduct under dark and anaerobic condition. Under the optimized production conditions, the titer of isopropanol was elevated 6-fold, to 2.42mM (146mg/l).

元の言語英語
ページ(範囲)585-590
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
119
発行部数5
DOI
出版物ステータス出版済み - 1 1 2015

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2-Propanol
Cyanobacteria
Metabolic Networks and Pathways
Solar Energy
Synechococcus
Alternative fuels
Carbon Dioxide
Solar energy
Byproducts
Carbon dioxide
Acetates
Light
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Optimization of isopropanol production by engineered cyanobacteria with a synthetic metabolic pathway. / Hirokawa, Yasutaka; Suzuki, Iwane; Hanai, Taizo.

:: Journal of Bioscience and Bioengineering, 巻 119, 番号 5, 01.01.2015, p. 585-590.

研究成果: ジャーナルへの寄稿記事

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