TY - JOUR
T1 - Optimization of lymphapheresis for manufacturing autologous CAR-T cells
AU - Yamanaka, Ikumi
AU - Yamauchi, Takuji
AU - Henzan, Tomoko
AU - Sakoda, Teppei
AU - Miyamoto, Kyoko
AU - Mishima, Hiroyuki
AU - Ono, Hiroaki
AU - Koga, Yuhki
AU - Nakashima, Yasuhiro
AU - Kato, Koji
AU - Miyamoto, Toshihiro
AU - Mizuno, Shinichi
AU - Ogawa, Yoshihiro
AU - Ohga, Shouichi
AU - Akashi, Koichi
AU - Maeda, Takahiro
AU - Kunisaki, Yuya
N1 - Funding Information:
We acknowledge the technical staffs at Center for Cellular and Molecular Medicine, Kyushu University Hospital for flow cytometric analysis and the clinical engineering technologists at Department of Medical Technology, Kyushu University Hospital for assistance with procedure of apheresis. We also thank the apheresis nurses at Blood Transfusion Center, Kyushu University Hospital for clinical care contributions.
Publisher Copyright:
© 2021, Japanese Society of Hematology.
PY - 2021/10
Y1 - 2021/10
N2 - Collection of CD3+ lymphocytes via lymphapheresis is essential for manufacturing autologous chimeric antigen receptor (CAR) T cells. Optimization of timing and procedures for lymphapheresis for each patient is critical because patients often have progressive diseases and receive medications that could reduce T cell counts. We conducted a retrospective study of clinical data from 28 patients who underwent lymphapheresis for CD19-directed CAR-T therapy with tisagenlecleucel to identify factors that could affect CD3+ lymphocyte yields. The numbers of CD3+ cells in peripheral blood were significantly correlated with CD3+ cell yields (correlation coefficient r = 0.84), which enabled us to estimate the volume of blood to process before apheresis. We also found that small cell ratio (SCR) at the apheresis site precisely reflected the proportion of lymphocytes, especially in patients without circulating blasts (coefficient of determination: r2 = 0.9). We were able to predict the CD3+ cell yield and prevent excessive apheresis by measuring pre-apheresis circulating CD3+ cell counts and monitoring SCR. Collectively, these results will help us to establish a strategy for optimization of lymphapheresis procedures for CAR-T cell production on a patient-by-patient basis.
AB - Collection of CD3+ lymphocytes via lymphapheresis is essential for manufacturing autologous chimeric antigen receptor (CAR) T cells. Optimization of timing and procedures for lymphapheresis for each patient is critical because patients often have progressive diseases and receive medications that could reduce T cell counts. We conducted a retrospective study of clinical data from 28 patients who underwent lymphapheresis for CD19-directed CAR-T therapy with tisagenlecleucel to identify factors that could affect CD3+ lymphocyte yields. The numbers of CD3+ cells in peripheral blood were significantly correlated with CD3+ cell yields (correlation coefficient r = 0.84), which enabled us to estimate the volume of blood to process before apheresis. We also found that small cell ratio (SCR) at the apheresis site precisely reflected the proportion of lymphocytes, especially in patients without circulating blasts (coefficient of determination: r2 = 0.9). We were able to predict the CD3+ cell yield and prevent excessive apheresis by measuring pre-apheresis circulating CD3+ cell counts and monitoring SCR. Collectively, these results will help us to establish a strategy for optimization of lymphapheresis procedures for CAR-T cell production on a patient-by-patient basis.
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U2 - 10.1007/s12185-021-03191-x
DO - 10.1007/s12185-021-03191-x
M3 - Article
C2 - 34275066
AN - SCOPUS:85110251957
SN - 0925-5710
VL - 114
SP - 449
EP - 458
JO - International Journal of Hematology
JF - International Journal of Hematology
IS - 4
ER -