Optimization of peptide density on microarray surface for quantitative phosphoproteomics

Takashi Shimomura, Xiaoming Han, Akito Hata, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Procedures to determine the density of peptides immobilized on a glass surface for the quantitative detection of phosphorylated peptides for phosphoproteomic applications of peptide microarrays are described. Two kinds of representative fluorescent probe molecules, anti-phosphotyrosine antibody (AB) and phos-tag (PT), were examined to compare their ability for the quantitative detection of phosphorylated peptides. PT is a metal complex with a binding specificity to phosphorylated amino acids, and is much smaller in size than AB. Thus, PT is quantitatively bound to the phosphorylated peptides, even at a high immobilization density without steric hindrance, making them highly suited for future microarrays requiring smaller sized peptide spots for much higher throughput. 2011

元の言語英語
ページ(範囲)13-17
ページ数5
ジャーナルanalytical sciences
27
発行部数1
DOI
出版物ステータス出版済み - 2011

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Microarrays
Peptides
Phosphotyrosine
Antibodies
Coordination Complexes
Fluorescent Dyes
Throughput
Amino Acids
Glass
Molecules
1,3-bis(bis(pyridin-2-ylmethyl)amino)propan-2-ol

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Optimization of peptide density on microarray surface for quantitative phosphoproteomics. / Shimomura, Takashi; Han, Xiaoming; Hata, Akito; Niidome, Takuro; Mori, Takeshi; Katayama, Yoshiki.

:: analytical sciences, 巻 27, 番号 1, 2011, p. 13-17.

研究成果: ジャーナルへの寄稿記事

Shimomura, Takashi ; Han, Xiaoming ; Hata, Akito ; Niidome, Takuro ; Mori, Takeshi ; Katayama, Yoshiki. / Optimization of peptide density on microarray surface for quantitative phosphoproteomics. :: analytical sciences. 2011 ; 巻 27, 番号 1. pp. 13-17.
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