Organelle-localizable fluorescent chemosensors for site-specific multicolor imaging of nucleoside polyphosphate dynamics in living cells

Yasutaka Kurishita, Takahiro Kohira, Akio Ojida, Itaru Hamachi

研究成果: ジャーナルへの寄稿記事

88 引用 (Scopus)

抄録

ATP and its derivatives (nucleoside polyphosphates (NPPs)) are implicated in many biological events, so their rapid and convenient detection is important. In particular, live cell detection of NPPs at specific local regions of cells could greatly contribute understanding of the complicated roles of NPPs. We report herein the design of two new fluorescent chemosensors that detect the dynamics of NPPs in specific regions of living cells. To achieve imaging of NPPs on plasma membrane surfaces (2-2Zn(II)), a lipid anchor was introduced into xanthene-based Zn(II) complex 1-2Zn(II), which was previously developed as a turn-on type fluorescent chemosensor for NPPs. Meanwhile, for subcellular imaging of ATP in mitochondria, we designed rhodamine-type Zn(II) complex 3-2Zn(II), which possesses a cationic pyronin ring instead of xanthene. Detailed spectroscopic studies revealed that 2-2Zn(II) and 3-2Zn(II) can sense NPPs with a several-fold increase of their fluorescence intensities through a sensing mechanism similar to 1-2Zn(II), involving binding-induced recovery of the conjugated form of the xanthene or pyronin ring. In live cell imaging, 2-2Zn(II) containing a lipid anchor selectively localized on the plasma membrane surface and detected the extracellular release of NPPs during cell necrosis induced by streptolysin O. On the other hand, rhodamine-type complex 3-2Zn(II) spontaneously localized at mitochondria inside cells, and sensed the local increase of ATP concentration during apoptosis. Multicolor images were obtained through simultaneous use of 2-2Zn(II) and 3-2Zn(II), allowing detection of the dynamics of ATP in different cellular compartments at the same time.

元の言語英語
ページ(範囲)18779-18789
ページ数11
ジャーナルJournal of the American Chemical Society
134
発行部数45
DOI
出版物ステータス出版済み - 11 14 2012

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Polyphosphates
Nucleosides
Organelles
Cells
Imaging techniques
Xanthenes
Adenosinetriphosphate
Adenosine Triphosphate
Mitochondria
Rhodamines
Cell membranes
Anchors
Lipids
Cell Membrane
Cell death
Necrosis
Fluorescence
Apoptosis
Derivatives
Recovery

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

これを引用

Organelle-localizable fluorescent chemosensors for site-specific multicolor imaging of nucleoside polyphosphate dynamics in living cells. / Kurishita, Yasutaka; Kohira, Takahiro; Ojida, Akio; Hamachi, Itaru.

:: Journal of the American Chemical Society, 巻 134, 番号 45, 14.11.2012, p. 18779-18789.

研究成果: ジャーナルへの寄稿記事

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