Organization and nucleotide sequence of the DNA polymerase gene from the archaeon pyrococcus furiosus

Takashi Uemori, Yoshizumi Ishino, Hiroyuki Toh, Kiyozo Asada, Ikunoshin Kato

研究成果: Contribution to journalArticle

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We cloned the gene encoding the thermostable DNA polymerase from the archaeon Pyrococcus furiosus. The DNA fragment of 2785 base pair (bp) containing the structural gene for DNA polymerase was sequenced. DNA polymerase (Pfu polymerase), as deduced from the DNA sequence, consisted of 775 amlno acids, had a molecular weight of 90,109, and was structurally homologous to the α-like DNA polymerases (family B) represented by human DNA polymerase a and Escherichia coll DNA polymerase II. An unrooted phylogenetic tree of the α-like DNA polymerases based on the amino acid sequence alignment was constructed. Pfu polymerase, with two other archaeon polymerases, constitutes a group with some animal viruses. The transcription initiation sites of the pol gene were identified by analysis of In vivo transcripts of both from P.furiosus and E.coli, and the promoters were assigned upstream of the pol coding region. A typical promoter sequence for the archaeon was found at a reasonable distance from the transcription initiation site in P.furiosus.

元の言語英語
ページ(範囲)259-265
ページ数7
ジャーナルNucleic acids research
21
発行部数2
DOI
出版物ステータス出版済み - 1 25 1993
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Genetics

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