Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor β chain

B. Toyonaga, Y. Yoshikai, V. Vadasz, B. Chin, T. W. Mak

    研究成果: ジャーナルへの寄稿記事

    308 引用 (Scopus)

    抄録

    The organization and sequences of the human β-chain T-cell receptor diversity, joining, and constant region segments are described. The β chain of the human T-cell receptor, analogous to the mouse counterpart, consists of two distinct constant region genes ~ 10 kilobases apart. The two constant region genes, C(β1) and C(β2), are very similar not only in sequence but also in genomic organization. The coding sequences of each of these C(β) constant region genes are divided into four exons. The first two exons encode most of the extracellular constant domain. The third exon encodes a major part of the presumed transmembrane portion, and the last exon contains the cytoplasmic coding sequence as well as 3' untranslated sequences. Except for a stretch of ~ 95 highly conserved nucleotides extending 3' of the first exon of the C region genes, little homology can be found between the intron sequences of C(β1) and C(β2). A small cluster of joining region (J(β)) gene segments is located ~ 5 kilobases upstream of each of these two constant regions. The first cluster, J(β1), contains six functional J gene segments while the second, J(β2), contains seven functional J gene segments. In addition, diversity region (D(β)) gene segments are located ~ 600 base pairs upstream of each J(β). Recombinational signals containing higher conserved heptamer and nonamer sequences separated by 12 or 23 bases are found adjacent to all of these D(β) and J(β) gene segments. These signal sequences are thought to be involved in the somatic recombination processes. These results indicate that what appears to be a gene duplication event giving rise to these two distinct regions must have arisen a long time ago in the evolution of this gene locus.

    元の言語英語
    ページ(範囲)8624-8628
    ページ数5
    ジャーナルProceedings of the National Academy of Sciences of the United States of America
    82
    発行部数24
    DOI
    出版物ステータス出版済み - 1 1 1985

    Fingerprint

    T-Cell Receptor Genes
    Genes
    Exons
    T-Cell Antigen Receptor
    Gene Duplication
    Protein Sorting Signals
    Base Pairing
    Introns
    Genetic Recombination
    Nucleotides

    All Science Journal Classification (ASJC) codes

    • General

    これを引用

    Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor β chain. / Toyonaga, B.; Yoshikai, Y.; Vadasz, V.; Chin, B.; Mak, T. W.

    :: Proceedings of the National Academy of Sciences of the United States of America, 巻 82, 番号 24, 01.01.1985, p. 8624-8628.

    研究成果: ジャーナルへの寄稿記事

    @article{bc43df4fe8c542cc9dcc59553f06c4d6,
    title = "Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor β chain",
    abstract = "The organization and sequences of the human β-chain T-cell receptor diversity, joining, and constant region segments are described. The β chain of the human T-cell receptor, analogous to the mouse counterpart, consists of two distinct constant region genes ~ 10 kilobases apart. The two constant region genes, C(β1) and C(β2), are very similar not only in sequence but also in genomic organization. The coding sequences of each of these C(β) constant region genes are divided into four exons. The first two exons encode most of the extracellular constant domain. The third exon encodes a major part of the presumed transmembrane portion, and the last exon contains the cytoplasmic coding sequence as well as 3' untranslated sequences. Except for a stretch of ~ 95 highly conserved nucleotides extending 3' of the first exon of the C region genes, little homology can be found between the intron sequences of C(β1) and C(β2). A small cluster of joining region (J(β)) gene segments is located ~ 5 kilobases upstream of each of these two constant regions. The first cluster, J(β1), contains six functional J gene segments while the second, J(β2), contains seven functional J gene segments. In addition, diversity region (D(β)) gene segments are located ~ 600 base pairs upstream of each J(β). Recombinational signals containing higher conserved heptamer and nonamer sequences separated by 12 or 23 bases are found adjacent to all of these D(β) and J(β) gene segments. These signal sequences are thought to be involved in the somatic recombination processes. These results indicate that what appears to be a gene duplication event giving rise to these two distinct regions must have arisen a long time ago in the evolution of this gene locus.",
    author = "B. Toyonaga and Y. Yoshikai and V. Vadasz and B. Chin and Mak, {T. W.}",
    year = "1985",
    month = "1",
    day = "1",
    doi = "10.1073/pnas.82.24.8624",
    language = "English",
    volume = "82",
    pages = "8624--8628",
    journal = "Proceedings of the National Academy of Sciences of the United States of America",
    issn = "0027-8424",
    number = "24",

    }

    TY - JOUR

    T1 - Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor β chain

    AU - Toyonaga, B.

    AU - Yoshikai, Y.

    AU - Vadasz, V.

    AU - Chin, B.

    AU - Mak, T. W.

    PY - 1985/1/1

    Y1 - 1985/1/1

    N2 - The organization and sequences of the human β-chain T-cell receptor diversity, joining, and constant region segments are described. The β chain of the human T-cell receptor, analogous to the mouse counterpart, consists of two distinct constant region genes ~ 10 kilobases apart. The two constant region genes, C(β1) and C(β2), are very similar not only in sequence but also in genomic organization. The coding sequences of each of these C(β) constant region genes are divided into four exons. The first two exons encode most of the extracellular constant domain. The third exon encodes a major part of the presumed transmembrane portion, and the last exon contains the cytoplasmic coding sequence as well as 3' untranslated sequences. Except for a stretch of ~ 95 highly conserved nucleotides extending 3' of the first exon of the C region genes, little homology can be found between the intron sequences of C(β1) and C(β2). A small cluster of joining region (J(β)) gene segments is located ~ 5 kilobases upstream of each of these two constant regions. The first cluster, J(β1), contains six functional J gene segments while the second, J(β2), contains seven functional J gene segments. In addition, diversity region (D(β)) gene segments are located ~ 600 base pairs upstream of each J(β). Recombinational signals containing higher conserved heptamer and nonamer sequences separated by 12 or 23 bases are found adjacent to all of these D(β) and J(β) gene segments. These signal sequences are thought to be involved in the somatic recombination processes. These results indicate that what appears to be a gene duplication event giving rise to these two distinct regions must have arisen a long time ago in the evolution of this gene locus.

    AB - The organization and sequences of the human β-chain T-cell receptor diversity, joining, and constant region segments are described. The β chain of the human T-cell receptor, analogous to the mouse counterpart, consists of two distinct constant region genes ~ 10 kilobases apart. The two constant region genes, C(β1) and C(β2), are very similar not only in sequence but also in genomic organization. The coding sequences of each of these C(β) constant region genes are divided into four exons. The first two exons encode most of the extracellular constant domain. The third exon encodes a major part of the presumed transmembrane portion, and the last exon contains the cytoplasmic coding sequence as well as 3' untranslated sequences. Except for a stretch of ~ 95 highly conserved nucleotides extending 3' of the first exon of the C region genes, little homology can be found between the intron sequences of C(β1) and C(β2). A small cluster of joining region (J(β)) gene segments is located ~ 5 kilobases upstream of each of these two constant regions. The first cluster, J(β1), contains six functional J gene segments while the second, J(β2), contains seven functional J gene segments. In addition, diversity region (D(β)) gene segments are located ~ 600 base pairs upstream of each J(β). Recombinational signals containing higher conserved heptamer and nonamer sequences separated by 12 or 23 bases are found adjacent to all of these D(β) and J(β) gene segments. These signal sequences are thought to be involved in the somatic recombination processes. These results indicate that what appears to be a gene duplication event giving rise to these two distinct regions must have arisen a long time ago in the evolution of this gene locus.

    UR - http://www.scopus.com/inward/record.url?scp=1842340825&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=1842340825&partnerID=8YFLogxK

    U2 - 10.1073/pnas.82.24.8624

    DO - 10.1073/pnas.82.24.8624

    M3 - Article

    C2 - 3866244

    AN - SCOPUS:1842340825

    VL - 82

    SP - 8624

    EP - 8628

    JO - Proceedings of the National Academy of Sciences of the United States of America

    JF - Proceedings of the National Academy of Sciences of the United States of America

    SN - 0027-8424

    IS - 24

    ER -