Organization of nucleosides supported by boronic-acid-appended poly(L-lysine): Creation of a novel RNA mimic

H. Kobayashi, M. Amaike, K. Koumoto, S. Shinkai

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

Boronic-acid-appended poly(L-lysine) (1) can form anionic boronate esters with diol derivatives in aqueous solution. One can thus expect that when nucleosides are mixed with 1, the resultant complexes will satisfy the basic structural characteristics of RNA, viz., one-dimensionally organized nucleic bases and anionic charges. To obtain insights into this novel RNA mimic, the solution properties of adenosine (Ad) were estimated in the absence and the presence of 1. The large absorption and CD spectral changes were observed at pH 6-10 where the poly(L-lysine) main chain tends to adopt an α-helix-rich conformation. On the basis of the fact that the magnitude of the spectral changes is much larger than the fraction of complexed Ad molecules, the polymer-supported organization mode was proposed: that is, Ad molecules complexed by covalent bonds with 1 act as clusters to induce further organization of uncomplexed Ad molecules by noncovalent interactions. The proposal was further confirmed by 1H NMR spectroscopy and dynamic light scattering. On the other hand, none of these spectral changes were observed for 2′-deoxyadenosine. The 1-Ad complex thus formed can interact with complementary poly(U) but not with noncomplementary poly(C), indicating that it can act as a novel RNA mimic.

本文言語英語
ページ(範囲)1311-1317
ページ数7
ジャーナルBulletin of the Chemical Society of Japan
74
7
DOI
出版ステータス出版済み - 7 1 2001
外部発表はい

All Science Journal Classification (ASJC) codes

  • 化学 (全般)

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