Organization of the NKEF gene and its expression in the common carp (Cyprinus carpio)

Dong Ho Shin, Kazuhiro Fujiki, Miki Nakao, Tomoki Yano

研究成果: Contribution to journalArticle査読

35 被引用数 (Scopus)

抄録

Natural killer enhancing factor (NKEF) is a member of the newly defined peroxiredoxin (Prx) family. Its functions are to enhance the cytotoxic capacity of natural killer cells and to prevent DNA and protein from being damaged by oxidative stress in the presence of thiol compounds. However, little is known about the structure and function of NKEF in lower vertebrates. We have recently cloned a cDNA encoding NKEF from the common carp (Cyprinus carpio) by use of suppression subtractive hybridization (SSH). In the present study, we used PCR to obtain a genomic DNA which covers the entire coding region of carp NKEF. In the 3363bp-long genomic sequence, six exons and five introns were identified. The carp NKEF gene has splice donor/acceptor site sequences at the boundaries of exons and introns, and contains two Val-Cys-Pro (VCP) motifs. The exon/intron organization of the carp NKEF gene shows complete conservation with other members of the Prx family. Genomic Southern blotting analyses suggest that carp has multiple copies of the NKEF gene. RT-PCR analyses reveal that carp NKEF has very different expression levels not only in tissues but also from individuals.

本文言語英語
ページ(範囲)597-606
ページ数10
ジャーナルDevelopmental and Comparative Immunology
25
7
DOI
出版ステータス出版済み - 2001

All Science Journal Classification (ASJC) codes

  • 免疫学
  • 発生生物学

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