Organoid culture of human cancer stem cells

Yohei Shimono, Junko Mukohyama, Taichi Isobe, Darius M. Johnston, Piero Dalerba, Akira Suzuki

研究成果: Chapter in Book/Report/Conference proceedingChapter

5 被引用数 (Scopus)

抄録

Organoid culture is a three-dimensional culture method that enables ex vivo analysis of stem cell behavior and differentiation. This method is also applicable to the studies on stem cell characters of human cancer stem cells. The components of organoid culture include Matrigel® and a culture medium containing growth factor cocktails that mimic the microenvironments of organ stem cell niches. Here, we describe the basic methods for the organoid culture of dissociated or FACS-sorted human cancer stem cells. Then, we introduce a method to dissociate the organoids for serial passage and propagation.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ23-31
ページ数9
DOI
出版ステータス出版済み - 2019
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1576
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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