抄録
The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 1025-1028 |
| ページ数 | 4 |
| ジャーナル | Key Engineering Materials |
| 巻 | 361-363 II |
| 出版物ステータス | 出版済み - 1 22 2008 |
| 外部発表 | Yes |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Mechanics of Materials
- Mechanical Engineering
これを引用
Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. / Kumagai, Takashi; Anada, Takahisa; Honda, Yoshitomo; Takami, Masamichi; Kamijo, Ryutaro; Shimauchi, Hidetoshi; Suzuki, Osamu.
：: Key Engineering Materials, 巻 361-363 II, 22.01.2008, p. 1025-1028.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite
AU - Kumagai, Takashi
AU - Anada, Takahisa
AU - Honda, Yoshitomo
AU - Takami, Masamichi
AU - Kamijo, Ryutaro
AU - Shimauchi, Hidetoshi
AU - Suzuki, Osamu
PY - 2008/1/22
Y1 - 2008/1/22
N2 - The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.
AB - The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.
UR - http://www.scopus.com/inward/record.url?scp=38149104640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38149104640&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:38149104640
VL - 361-363 II
SP - 1025
EP - 1028
JO - Key Engineering Materials
JF - Key Engineering Materials
SN - 1013-9826
ER -