Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite

Takashi Kumagai, Takahisa Anada, Yoshitomo Honda, Masamichi Takami, Ryutaro Kamijo, Hidetoshi Shimauchi, Osamu Suzuki

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

元の言語英語
ページ(範囲)1025-1028
ページ数4
ジャーナルKey Engineering Materials
361-363 II
出版物ステータス出版済み - 1 22 2008
外部発表Yes

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Bone Morphogenetic Protein 2
Durapatite
Hydroxyapatite
Bone
Phosphates
Proteins
Calcium phosphate
Calcium Phosphates
Adsorption
octacalcium phosphate
Temperature
Cells
Plastics
Coatings

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

これを引用

Kumagai, T., Anada, T., Honda, Y., Takami, M., Kamijo, R., Shimauchi, H., & Suzuki, O. (2008). Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. Key Engineering Materials, 361-363 II, 1025-1028.

Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. / Kumagai, Takashi; Anada, Takahisa; Honda, Yoshitomo; Takami, Masamichi; Kamijo, Ryutaro; Shimauchi, Hidetoshi; Suzuki, Osamu.

:: Key Engineering Materials, 巻 361-363 II, 22.01.2008, p. 1025-1028.

研究成果: ジャーナルへの寄稿記事

Kumagai, T, Anada, T, Honda, Y, Takami, M, Kamijo, R, Shimauchi, H & Suzuki, O 2008, 'Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite', Key Engineering Materials, 巻. 361-363 II, pp. 1025-1028.
Kumagai T, Anada T, Honda Y, Takami M, Kamijo R, Shimauchi H その他. Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. Key Engineering Materials. 2008 1 22;361-363 II:1025-1028.
Kumagai, Takashi ; Anada, Takahisa ; Honda, Yoshitomo ; Takami, Masamichi ; Kamijo, Ryutaro ; Shimauchi, Hidetoshi ; Suzuki, Osamu. / Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. :: Key Engineering Materials. 2008 ; 巻 361-363 II. pp. 1025-1028.
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