Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Ryuhei Nishikawa, Takahisa Anada, Risa Ishiko-Uzuka, Osamu Suzuki

研究成果: ジャーナルへの寄稿学術誌査読

15 被引用数 (Scopus)

抄録

The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property.

本文言語英語
ページ(範囲)242-251
ページ数10
ジャーナルdental materials journal
33
2
DOI
出版ステータス出版済み - 2014
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • セラミックおよび複合材料
  • 歯科学(全般)

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