Osteoblasts and Osteocytes Express MMP2 and -8 and TIMP1, -2, and -3 Along with Extracellular Matrix Molecules during Appositional Bone Formation

Kouki Hatori, Yasuyuki Sasano, Ichiro Takahashi, Shinji Kamakura, Manabu Kagayama, Keiichi Sasaki

研究成果: Contribution to journalArticle査読

38 被引用数 (Scopus)

抄録

Our previous studies suggested that a part of bone extracellular matrix (ECM) molecules are degraded and remodeled during embryonic bone formation. In contrast, little is known about ECM remodeling in postnatal appositional bone formation. The present study was designed to investigate expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) during experimentally initiated appositional bone formation in rats. Expressions of ECM molecules, MMPs, and TIMPs were examined using in situ hybridization. Osteoblasts and osteocytes expressed MMP2 and -8, TIMP1, -2, and -3, as well as type I collagen, osteopontin, and osteocalcin in the course of the appositional bone formation, while they showed few transcripts of MMP13. The results indicated that while osteoblasts and osteocytes in the apposed bone produce ECM molecules, they degrade ECM molecules with MMPs and regulate the degradation by inhibiting the activity of MMPs using TIMPs. Osteoblasts and osteocytes may reorganize the ECM composition to mature the bone matrix in appositional bone formation.

本文言語英語
ページ(範囲)262-271
ページ数10
ジャーナルAnatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
277
2
DOI
出版ステータス出版済み - 4 2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 解剖学
  • 農業および生物科学(その他)

フィンガープリント

「Osteoblasts and Osteocytes Express MMP2 and -8 and TIMP1, -2, and -3 Along with Extracellular Matrix Molecules during Appositional Bone Formation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル