Overexpression of eNOS in NTS causes hypotension and bradycardia in vivo

Koji Sakai, Yoshitaka Hirooka, Isamu Matsuo, Kenichi Eshima, Hideaki Shigematsu, Hiroaki Shimokawa, Akira Takeshita

研究成果: Contribution to journalArticle査読

92 被引用数 (Scopus)

抄録

The role of nitric oxide (NO) in the brain in the control of blood pressure and the sympathetic nervous system is debated. This study examined the effect of overexpression of endothelial NO synthase (eNOS) in the nucleus tractus solitarii (NTS) on blood pressure in conscious rats. Adenovirus vectors encoding either eNOS (AdeNOS) or β-galactosidase were transfected into the NTS in vivo. In the AdeNOS-treated rats, the local expression of eNOS in the NTS was confirmed by immunohistochemical staining and Western blot analysis for the eNOS protein and by increased production of nitrite/nitrate in the NTS measured by in vivo microdialysis. Blood pressure and heart rate, monitored by the use of a radiotelemetry system in a conscious state, were significantly decreased in the AdeNOS-treated group at day 5 to day 10 after the gene transfer. Urinary norepinephrine excretion also was decreased at day 7 after the gene transfer in the AdeNOS-treated group. Our results indicate that overexpression of eNOS in the NTS decreases blood pressure, heart rate, and sympathetic nerve activity in conscious rats.

本文言語英語
ページ(範囲)1023-1028
ページ数6
ジャーナルHypertension
36
6
DOI
出版ステータス出版済み - 2000

All Science Journal Classification (ASJC) codes

  • 内科学

フィンガープリント

「Overexpression of eNOS in NTS causes hypotension and bradycardia in vivo」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル