Overexpression of PDR16 confers resistance to complex sphingolipid biosynthesis inhibitor aureobasidin A in yeast Saccharomyces cerevisiae

Yuka Katsuki, Yutaro Yamaguchi, Motohiro Tani

研究成果: ジャーナルへの寄稿記事

抄録

Sphingolipids are essential for normal cell growth of yeast Saccharomyces cerevisiae. Aureobasidin A (AbA), an antifungal drug, inhibits Aur1, an enzyme catalyzing the synthesis of inositol phosphorylceramide, and induces a strong growth defect in yeast. In this study, we screened for multicopy suppressor genes that confer resistance to AbA, and identified PDR16. In addition, it was found that PDR17, a paralog of PDR16, also functions as a multicopy suppressor. Pdr16 and Pdr17 belong to a family of phosphatidylinositol transfer proteins; however, cells overexpressing the other members of the family hardly exhibited resistance to AbA. Overexpression of a lipid-binding defective mutant of Pdr16 did not confer the resistance to AbA, indicating that the lipid-binding activity is essential for acquiring resistance to AbA. When expression of the AUR1 gene was repressed by a tetracycline-regulatable promoter, the overexpression of PDR16 or PDR17 did not suppress the growth defect caused by the AUR1 repression. Quantification analysis of complex sphingolipids revealed that in AbA-treated cells, but not in cells in which AUR1 was repressed by the tetracycline-regulatable promoter, the reductions of complex sphingolipid levels were suppressed by the overexpressed PDR16. Thus, it was indicated that the overexpression of PDR16 reduces the effectiveness of AbA against intracellular Aur1 activity.

元の言語英語
記事番号fnx255
ジャーナルFEMS microbiology letters
365
発行部数3
DOI
出版物ステータス出版済み - 2 1 2018

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Sphingolipids
Saccharomyces cerevisiae
Yeasts
Tetracycline
Growth
Phospholipid Transfer Proteins
Suppressor Genes
Lipids
Inositol
aureobasidin A
Gene Expression
Enzymes
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

これを引用

Overexpression of PDR16 confers resistance to complex sphingolipid biosynthesis inhibitor aureobasidin A in yeast Saccharomyces cerevisiae. / Katsuki, Yuka; Yamaguchi, Yutaro; Tani, Motohiro.

:: FEMS microbiology letters, 巻 365, 番号 3, fnx255, 01.02.2018.

研究成果: ジャーナルへの寄稿記事

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