Overexpression of the wild type p73 gene in human bladder cancer

Akira Yokomizo, Ming Mai, Donald J. Tindall, Liang Cheng, David G. Bostwick, Seiji Naito, David I. Smith, Wanguo Liu

研究成果: ジャーナルへの寄稿学術誌査読

125 被引用数 (Scopus)

抄録

p73, a first p53 relative, was recently identified and shown to be monoallelically expressed in a number of different human tissues. To determine the potential role of this gene in human bladder cancer, we investigated p73 expression levels, allelic expression patterns, and analysed p73 mutations in 23 unselected primary invasive bladder cancers with matched normal tissues and in seven bladder cancer cell lines. In a comparison between normal and tumor tissues using quantitative RT-PCR analysis, we found that p73 was overexpressed in 22/23 bladder cancers, sometimes as great as 20-fold. Allelic expression analysis using a C/T polymorphism in exon 2 and a newly identified T/C polymorphism in exon 5 revealed that p73 was biallelically expressed in both normal bladder and cancer tissues, suggesting that p73 is not imprinted in bladder tissue. Mutation screening of the p73 gene in bladder cancer DNAs using denaturing high-performance liquid chromatography analysis and DNA sequencing revealed no tumor-specific mutations in any coding exons of the p73 gene. These data suggest that the p73 is unlikely to be a tumor suppressor gene, but that overexpression of p73 may contribute to tumorigenesis in bladder cancer.

本文言語英語
ページ(範囲)1629-1633
ページ数5
ジャーナルOncogene
18
8
DOI
出版ステータス出版済み - 2月 25 1999
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学
  • 癌研究

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