Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus

Hirokazu Nishida, Daisuke Tsuchiya, Yoshizumi Ishino, Kosuke Morikawa

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase. Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution. They belong to the monoclinic space group P21, with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 Å, β = 108.9°. The asymmetric unit contains one ligase molecule.

本文言語英語
ページ(範囲)1100-1102
ページ数3
ジャーナルActa Crystallographica Section F: Structural Biology and Crystallization Communications
61
12
DOI
出版ステータス出版済み - 2005

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 遺伝学
  • 凝縮系物理学

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