TY - JOUR
T1 - Overlapping distributions of mammalian types I, II, and III taste cell markers in chicken taste buds
AU - Yoshida, Yuta
AU - Kawabata, Fuminori
AU - Nishimura, Shotaro
AU - Tabata, Shoji
N1 - Funding Information:
This study was supported by grants to F. Kawabata from JSPS KAKENHI ( #17K08047 and #19KT0005 ), a grant to S. Tabata from the JSPS KAKENHI ( #18H02330 ), and grants to Y. Yoshida from the JSPS Research Fellowship ( #17J03180 ) and the Kieikai Research Foundation (# 2020C022 ).
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/9/17
Y1 - 2021/9/17
N2 - Mammalian taste buds comprise types I, II, and III taste cells, with each type having specific characteristics: glia-like supporting cells (type I), taste receptor cells (type II), and presynaptic cells (type III). In this study, to characterize the peripheral taste-sensing systems in chickens, we analyzed the distributions of the mammalian types I, II, and III taste cell markers in chicken taste buds: glutamate-aspartate transporter (GLAST) for type I; taste receptor type 1 members 1 and 3 (T1R1 and T1R3), taste receptor type 2 member 7 (T2R7), and α-gustducin for type II; and synaptosomal protein 25 (SNAP25) and neural cell adhesion molecule (NCAM) for type III. We found that most GLAST+ taste cells expressed α-gustducin and SNAP25 and that high percentages of T1R3+ or α-gustducin+ taste cells expressed SNAP25 and NCAM. These results demonstrated a unique subset of chicken taste cells expressing multiple taste cell type marker proteins. Taken together, these results provide new insights into the taste-sensing mechanisms in vertebrate taste buds.
AB - Mammalian taste buds comprise types I, II, and III taste cells, with each type having specific characteristics: glia-like supporting cells (type I), taste receptor cells (type II), and presynaptic cells (type III). In this study, to characterize the peripheral taste-sensing systems in chickens, we analyzed the distributions of the mammalian types I, II, and III taste cell markers in chicken taste buds: glutamate-aspartate transporter (GLAST) for type I; taste receptor type 1 members 1 and 3 (T1R1 and T1R3), taste receptor type 2 member 7 (T2R7), and α-gustducin for type II; and synaptosomal protein 25 (SNAP25) and neural cell adhesion molecule (NCAM) for type III. We found that most GLAST+ taste cells expressed α-gustducin and SNAP25 and that high percentages of T1R3+ or α-gustducin+ taste cells expressed SNAP25 and NCAM. These results demonstrated a unique subset of chicken taste cells expressing multiple taste cell type marker proteins. Taken together, these results provide new insights into the taste-sensing mechanisms in vertebrate taste buds.
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U2 - 10.1016/j.bbrc.2021.07.025
DO - 10.1016/j.bbrc.2021.07.025
M3 - Article
C2 - 34284142
AN - SCOPUS:85110762807
VL - 570
SP - 162
EP - 168
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
ER -