Overproduction, crystallization and preliminary X-ray diffraction analysis of probable ATP sulfurylase from Thermus thermophilus HB8

Yuichi Taguchi, Jun Hoseki, Yoshimitsu Kakuta, Keiichi Fukuyama

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

ATP sulfurylase catalyzes the reaction of inorganic sulfate with ATP, producing adenosine-5′-phosphosulfate (APS) and pyrophosphate. A probable ATP sulfurylase (MW = 38.8 kDa) from Thermus thermophilus HB8 was overproduced in Escherichia coli and purified. It was crystallized in the presence of 5 mM APS by the batch method. The crystal was monoclinic, space group P2 1, with unit-cell parameters a = 68.8, b = 61.2, c = 128.6 Å, β = 95.4°. Diffraction to better than 2.5 Å resolution was obtained using synchrotron radiation at SPring-8. The asymmetric unit most probably contains two subunits (VM = 3.48 Å3 Da -1).

本文言語英語
ページ(範囲)1645-1647
ページ数3
ジャーナルActa Crystallographica - Section D Biological Crystallography
59
9
DOI
出版ステータス出版済み - 9 1 2003

All Science Journal Classification (ASJC) codes

  • 構造生物学

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