Overview: Engineering transgenic constructs and mice

Naoto Haruyama, Andrew Cho, Ashok B. Kulkarni

研究成果: Contribution to journalReview article査読

50 被引用数 (Scopus)

抄録

Cell biology research encompasses everything from single cells to whole animals. Recent discoveries concerning particular gene functions can be applied to the whole animal for understanding genotype-phenotype relationships underlying disease mechanisms. For this reason, genetically manipulated mousemodels are nowconsidered essential to correctly understand disease processes in whole animals. This unit reviews the basic mouse technologies used to generate conventional transgenic mice, which represent a gain-of-function approach. First, an overview of transgenic construct design is presented. This unit then explains basic strategies for the identification and establishment of independent transgenic mouse lines, followed by comments on historical and emerging techniques. It then describes typical problems that are encountered when researchers start to generate transgenic mice.

本文言語英語
ページ(範囲)19.10.1-19.10.9
ジャーナルCurrent Protocols in Cell Biology
SUPPL. 42
DOI
出版ステータス出版済み - 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 細胞生物学

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