Oxidative stress caused by a low concentration of hydrogen peroxide induces senescence-like changes in mouse gingival fibroblasts

Tamotsu Kiyoshima, Norio Enoki, Ieyoshi Kobayashi, Takako Sakai, Kengo Nagata, Hiroko Wada, Hiroaki Fujiwara, Yukiko Ookuma, Hidetaka Sakai

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

Periodontal tissue deteriorates under persistent oxidative stress induced by inflammatory reactions in the microflora of the oral cavity. This study aimed to evaluate the cellular properties of mouse gingival fibroblasts (MGFs) in the presence of oxidative stress. MGFs from 10-, 30- and 52-week-old mice were used to evaluate the changes in the cellular properties with aging. The study investigated the effects of oxidative stress on the cellular properties of MGFs from 10-week-old mice. The expression of p53, p21 and murine double minute 2 (Mdm2) in the MGFs in response to oxidative stress was also examined. By day 8, the number of MGFs increased in culture. However, the increase was markedly lower in MGFs derived from aged mice. Oxidative stress due to hydrogen peroxide (H2O2)-induced morphological changes characterized by a round shape with enlarged nuclei and expanded cytoplasm. The cell number of MGFs was decreased subsequent to treatment with 50 μM or a higher concentration of H2O2. MGFs treated with H2O2 at 20 μM showed a similar cell growth curve as the one seen in 52-week-old mice. Phosphorylated p53 protein was increased in MGFs subsequent to treatment with 20 μM H2O2, along with an upregulated transcription of p21 and Mdm2 mRNAs. These results suggest that treatment with a lower concentration of H2O2 in MGFs induces cell cycle arrest, resulting in stress-induced premature senescence, possibly correlated with the development of periodontal diseases.

元の言語英語
ページ(範囲)1007-1012
ページ数6
ジャーナルInternational journal of molecular medicine
30
発行部数5
DOI
出版物ステータス出版済み - 11 1 2012

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Hydrogen Peroxide
Oxidative Stress
Fibroblasts
Periodontal Diseases
Cell Cycle Checkpoints
Mouth
Cytoplasm
Cell Count

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Oxidative stress caused by a low concentration of hydrogen peroxide induces senescence-like changes in mouse gingival fibroblasts. / Kiyoshima, Tamotsu; Enoki, Norio; Kobayashi, Ieyoshi; Sakai, Takako; Nagata, Kengo; Wada, Hiroko; Fujiwara, Hiroaki; Ookuma, Yukiko; Sakai, Hidetaka.

:: International journal of molecular medicine, 巻 30, 番号 5, 01.11.2012, p. 1007-1012.

研究成果: ジャーナルへの寄稿記事

Kiyoshima, Tamotsu ; Enoki, Norio ; Kobayashi, Ieyoshi ; Sakai, Takako ; Nagata, Kengo ; Wada, Hiroko ; Fujiwara, Hiroaki ; Ookuma, Yukiko ; Sakai, Hidetaka. / Oxidative stress caused by a low concentration of hydrogen peroxide induces senescence-like changes in mouse gingival fibroblasts. :: International journal of molecular medicine. 2012 ; 巻 30, 番号 5. pp. 1007-1012.
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abstract = "Periodontal tissue deteriorates under persistent oxidative stress induced by inflammatory reactions in the microflora of the oral cavity. This study aimed to evaluate the cellular properties of mouse gingival fibroblasts (MGFs) in the presence of oxidative stress. MGFs from 10-, 30- and 52-week-old mice were used to evaluate the changes in the cellular properties with aging. The study investigated the effects of oxidative stress on the cellular properties of MGFs from 10-week-old mice. The expression of p53, p21 and murine double minute 2 (Mdm2) in the MGFs in response to oxidative stress was also examined. By day 8, the number of MGFs increased in culture. However, the increase was markedly lower in MGFs derived from aged mice. Oxidative stress due to hydrogen peroxide (H2O2)-induced morphological changes characterized by a round shape with enlarged nuclei and expanded cytoplasm. The cell number of MGFs was decreased subsequent to treatment with 50 μM or a higher concentration of H2O2. MGFs treated with H2O2 at 20 μM showed a similar cell growth curve as the one seen in 52-week-old mice. Phosphorylated p53 protein was increased in MGFs subsequent to treatment with 20 μM H2O2, along with an upregulated transcription of p21 and Mdm2 mRNAs. These results suggest that treatment with a lower concentration of H2O2 in MGFs induces cell cycle arrest, resulting in stress-induced premature senescence, possibly correlated with the development of periodontal diseases.",
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AU - Nagata, Kengo

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AU - Ookuma, Yukiko

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