Oxygen concentration dependence of lipid peroxidation and lipid-derived radical generation: Application of profluorescent nitroxide switch

Fumiya Mito, Kana Kitagawa, Toshihide Yamasaki, Chisato Shirahama, Taketoshi Oishi, Yuko Ito, Mayumi Yamato, Ken Ichi Yamada

研究成果: ジャーナルへの寄稿学術誌査読

11 被引用数 (Scopus)

抄録

Lipid-derived radicals and peroxides are involved in the pathogenesis of oxidative stress diseases and, although lipid peroxide production is a required reaction between a lipid radical and molecular oxygen, a useful lipid radical detection method has remained tentative. Also, the effect of oxygen concentration on lipid peroxide production must be considered because of the hypoxic conditions in cancer and ischemic regions. In this study, the focus was on nitroxide reactivity, which allows spin trapping with carbon-centred radicals via radicalradical reactions and fluorophore quenching through interactions with nitroxide's unpaired electron. Thus, the aim here was to demonstrate a useful detection method for lipid-derived radicals as well as to clarify the effects of oxygen concentration on lipid peroxide production using profluorescent nitroxide. This latter compound reacted with lipid-derived radicals in a manner inversely dependent on oxygen concentration, resulting in fluorescence due to alkoxyamine formation and, conversely, lipid peroxide concentrations decreased with lower oxygen in the reaction system. Furthermore, nitroxide inhibited lipid peroxide production and stopped oxygen consumption in the same solution. These results suggested that the novel application of profluorescent nitroxide could directly and sensitively detect lipid-derived radicals and that radical and peroxide production were dependent on oxygen concentration.

本文言語英語
ページ(範囲)1103-1110
ページ数8
ジャーナルFree Radical Research
45
9
DOI
出版ステータス出版済み - 9月 2011

!!!All Science Journal Classification (ASJC) codes

  • 生化学

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