P2Y12 receptors in primary microglia activate nuclear factor of activated T-cell signaling to induce C–C chemokine 3 expression

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Microglia are widely accepted as surveillants in the central nervous system that are continually searching the local environment for signs of injury. Following an inflammatory situation, microglia alter their morphology, extend ramified processes, and undergo cell body hypertrophy. Extracellular nucleotides are recognized as a danger signal by microglia. ADP acting on P2Y12 receptors induce process extension of microglia thereby attracting microglia to the site of adenosine tri-phosphate/ADP leaking or release. However, the question whether ADP/P2Y12 receptor signaling directly stimulates the production or release of inducible factors such as cytokines remains unclear. In this study, we found that CC chemokine ligand 3 (CCL3) is induced by ADP-treated primary microglia. Pharmacological characterization using pertussis toxin, a P2Y12 receptor inhibitor, and a calcium chelator revealed that CCL3 induction was caused by P2Y12 receptor-mediated intracellular calcium elevation. Next, nuclear factor of activated T-cell dephosphorylation and nuclear translocalization were observed. Calcineurin, an inhibitor for nuclear factor of activated T cell, suppressed CCL3 induction. These data indicate that microglial P2Y12 receptors are utilized to trigger an acute inflammatory response in microglia via rapid CCL3 induction after ADP stimulation. (Figure presented.).

元の言語英語
ページ(範囲)100-110
ページ数11
ジャーナルJournal of Neurochemistry
141
発行部数1
DOI
出版物ステータス出版済み - 4 1 2017

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NFATC Transcription Factors
Cell signaling
CC Chemokines
Microglia
Adenosine Diphosphate
Ligands
Purinergic P2 Receptors
Adenine Nucleotides
Pertussis Toxin
Neurology
Adenosine
Hypertrophy
Nucleotides
Central Nervous System
Phosphates
Cells
Pharmacology
Cytokines
Calcium
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

これを引用

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title = "P2Y12 receptors in primary microglia activate nuclear factor of activated T-cell signaling to induce C–C chemokine 3 expression",
abstract = "Microglia are widely accepted as surveillants in the central nervous system that are continually searching the local environment for signs of injury. Following an inflammatory situation, microglia alter their morphology, extend ramified processes, and undergo cell body hypertrophy. Extracellular nucleotides are recognized as a danger signal by microglia. ADP acting on P2Y12 receptors induce process extension of microglia thereby attracting microglia to the site of adenosine tri-phosphate/ADP leaking or release. However, the question whether ADP/P2Y12 receptor signaling directly stimulates the production or release of inducible factors such as cytokines remains unclear. In this study, we found that CC chemokine ligand 3 (CCL3) is induced by ADP-treated primary microglia. Pharmacological characterization using pertussis toxin, a P2Y12 receptor inhibitor, and a calcium chelator revealed that CCL3 induction was caused by P2Y12 receptor-mediated intracellular calcium elevation. Next, nuclear factor of activated T-cell dephosphorylation and nuclear translocalization were observed. Calcineurin, an inhibitor for nuclear factor of activated T cell, suppressed CCL3 induction. These data indicate that microglial P2Y12 receptors are utilized to trigger an acute inflammatory response in microglia via rapid CCL3 induction after ADP stimulation. (Figure presented.).",
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AU - Tozaki-Saitoh, Hidetoshi

AU - Miyata, Hiroyuki

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AU - Matsushita, Katsuyuki

AU - Tsuda, Makoto

AU - Inoue, Kazuhide

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