p40phox as an alternative organizer to p47phox in Nox2 activation: A new mechanism involving an interaction with p22phox

Minoru Tamura, Iichiro Shiozaki, Shohei Ono, Kei Miyano, Sachio Kunihiro, Takayuki Sasaki

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

p40phox activated phagocyte NADPH oxidase without p47phox in a cell-free system consisting of p67phox, Rac and cytochrome b558 relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47phox. Addition of p47phox slightly increased the activation, but not additively. p40phox improved the efficiency of p67phox in the activation. The C-terminus-truncated p67phox, p40phox(D289A), p40phox(R58A), or p40phox(W207R) showed an impaired activation. A peptide corresponding to the p22phox Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40phox SH3 domain. Thus, p40phox can substitute for p47phox in the activation, interacting with p22phox and p67phox through their specific regions.

元の言語英語
ページ(範囲)4533-4538
ページ数6
ジャーナルFEBS Letters
581
発行部数23
DOI
出版物ステータス出版済み - 9 18 2007

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Chemical activation
Far-Western Blotting
src Homology Domains
Cell-Free System
NADPH Oxidase
Phagocytes
neutrophil cytosol factor 40K
Peptides
neutrophil cytosol factor 67K

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

p40phox as an alternative organizer to p47phox in Nox2 activation : A new mechanism involving an interaction with p22phox. / Tamura, Minoru; Shiozaki, Iichiro; Ono, Shohei; Miyano, Kei; Kunihiro, Sachio; Sasaki, Takayuki.

:: FEBS Letters, 巻 581, 番号 23, 18.09.2007, p. 4533-4538.

研究成果: ジャーナルへの寄稿記事

Tamura, Minoru ; Shiozaki, Iichiro ; Ono, Shohei ; Miyano, Kei ; Kunihiro, Sachio ; Sasaki, Takayuki. / p40phox as an alternative organizer to p47phox in Nox2 activation : A new mechanism involving an interaction with p22phox. :: FEBS Letters. 2007 ; 巻 581, 番号 23. pp. 4533-4538.
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title = "p40phox as an alternative organizer to p47phox in Nox2 activation: A new mechanism involving an interaction with p22phox",
abstract = "p40phox activated phagocyte NADPH oxidase without p47phox in a cell-free system consisting of p67phox, Rac and cytochrome b558 relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70{\%} of that by p47phox. Addition of p47phox slightly increased the activation, but not additively. p40phox improved the efficiency of p67phox in the activation. The C-terminus-truncated p67phox, p40phox(D289A), p40phox(R58A), or p40phox(W207R) showed an impaired activation. A peptide corresponding to the p22phox Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40phox SH3 domain. Thus, p40phox can substitute for p47phox in the activation, interacting with p22phox and p67phox through their specific regions.",
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T1 - p40phox as an alternative organizer to p47phox in Nox2 activation

T2 - A new mechanism involving an interaction with p22phox

AU - Tamura, Minoru

AU - Shiozaki, Iichiro

AU - Ono, Shohei

AU - Miyano, Kei

AU - Kunihiro, Sachio

AU - Sasaki, Takayuki

PY - 2007/9/18

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N2 - p40phox activated phagocyte NADPH oxidase without p47phox in a cell-free system consisting of p67phox, Rac and cytochrome b558 relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47phox. Addition of p47phox slightly increased the activation, but not additively. p40phox improved the efficiency of p67phox in the activation. The C-terminus-truncated p67phox, p40phox(D289A), p40phox(R58A), or p40phox(W207R) showed an impaired activation. A peptide corresponding to the p22phox Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40phox SH3 domain. Thus, p40phox can substitute for p47phox in the activation, interacting with p22phox and p67phox through their specific regions.

AB - p40phox activated phagocyte NADPH oxidase without p47phox in a cell-free system consisting of p67phox, Rac and cytochrome b558 relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47phox. Addition of p47phox slightly increased the activation, but not additively. p40phox improved the efficiency of p67phox in the activation. The C-terminus-truncated p67phox, p40phox(D289A), p40phox(R58A), or p40phox(W207R) showed an impaired activation. A peptide corresponding to the p22phox Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40phox SH3 domain. Thus, p40phox can substitute for p47phox in the activation, interacting with p22phox and p67phox through their specific regions.

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