p53 status-dependent sensitization of human tumour cells to hyperthermia by plant flavonol

Tomoyuki Hamamoto, Keiji Suzuki, Motohiro Yamauchi, Seiji Kodama, Hitoshi Sasaki, Masami Watanabe

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

Purpose: Quercetin (QCT), an important flavonol, is known to sensitize tumour cells to hyperthermia by suppressing heat shock protein 72 (Hsp72) induction, and is also reported to inhibit p53 accumulation. This study was conducted to examine the effects of QCT on the heat sensitivities of human tumour cell lines with different p53 statuses. Material and methods: Cell lines derived from human cancers and p53-inducible cells were used. After heat treatment at 43°C for 2 h with or without QCT, cell survival was determined in a clonogenic assay. The cellular and nuclear content of Hsp72 as well as that of p53 was determined by Western blotting analysis. Results: Treatment of cells with 150 μM QCT, which completely abolished Hsp72 induction, potentiated the lethal effects of hyperthermia in all tumour cell lines. Particularly, remarkable enhancement of cell death was observed in tumour cell lines having little or no p53 proteins. Although nuclear translocation of Hsp72 is induced by hyperthermia, it was significantly compromised in p53-deficient cells. Conclusions: These results indicate that p53 is a component for nuclear accumulation of Hsp72; therefore, p53 status is an important determinant of the sensitization of human tumour cells to hyperthermia by QCT.

本文言語英語
ページ(範囲)415-424
ページ数10
ジャーナルInternational Journal of Hyperthermia
24
5
DOI
出版ステータス出版済み - 2008
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 生理学(医学)
  • 癌研究

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