PACAP stimulates the release of interleukin-6 in cultured rat Müller cells

T. Seki, Y. Hinohara, C. Taki, M. Nakatani, M. Ozawa, S. Nishimura, A. Takaki, H. Itho, F. Takenoya, S. Shioda

研究成果: ジャーナルへの寄稿学術誌査読

19 被引用数 (Scopus)


We have investigated the in vivo effect of PACAP on rat Müller cells that are the predominant glial element in the retina. Müller cells were treated with PACAP38, either alone or in the presence of the PACAP-selective antagonist, PACAP6-38. Cellular proliferation was determined by measuring the incorporation of bromodeoxyuridine, while interleukin-6 (IL-6) levels in the culture medium were examined using a B9 cell bioassay. In cultured rat Müller cells, the expression of PACAP receptor (PAC1-R) was assessed with immunohistochemistry using a PAC1-R-specific antiserum. PACAP stimulated IL-6 production in Müller cells at a concentration as low as 10-12 M, which was not sufficient to induce cell proliferation. This elevation of IL-6 production was significantly inhibited by PACAP6-38. These data suggest that Müller cells are one of the target cells for PACAP, stimulating the release of IL-6, and providing a mechanism whereby PACAP exerts a significant neuroprotective effect in the retina.

ジャーナルAnnals of the New York Academy of Sciences
出版ステータス出版済み - 7月 2006

!!!All Science Journal Classification (ASJC) codes

  • 神経科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 科学史および科学哲学


「PACAP stimulates the release of interleukin-6 in cultured rat Müller cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。