Pancreastatin molecular forms in normal human plasma

Kitayama Noboru Kitayama, Tateishi Kayoko Tateishi, Funakoshi Akihiro Funakoshi, Miyasak Kyoko Miyasak, Shimazoe Takao Shimazoe, Kono Akira Kono, Iwamoto Naoko Iwamoto, Matsuoka Yuji Matsuoka

研究成果: Contribution to journalArticle査読

19 被引用数 (Scopus)

抄録

Circulating molecular forms with pancreastatin (PST)-like immunoreactivity in plasma from normal subjects were examined. An immunoreactive form corresponding to a human PST-like sequence [human chromogranin-A-(250-301)] (hPST-52) and a larger form (mol wt 15-21 kDa) were detected by gel filtration of plasma from normal subjects. On high performance liquid chromatography, predominant immunoreactive forms coeluted with the three larger forms which were purified from the xenograft of human pancreatic islet cell carcinoma cell line QGP-1N cells and with synthetic hPST-52. The fraction containing larger forms purified from xenograft of QGP-1N cells had biological activity equivalent to that of hPST-52 on the inhibition of pancreatic exocrine secretion. These results suggest that the larger molecular forms as well as hPST-52 may be physiologically important circulating forms of PST in human.

本文言語英語
ページ(範囲)1571-1578
ページ数8
ジャーナルLife Sciences
54
21
DOI
出版ステータス出版済み - 1994
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学、毒性学および薬学(全般)

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