Paraffin-embedded vertical sections of mouse embryonic stem cells

Hiroyuki Imai, Wataru Fujii, Ken Takeshi Kusakabe, Yasuo Kiso, Etsuro Ono, Kiyoshi Kano

研究成果: Contribution to journalComment/debate査読

抄録

Cultured cells are generally observed through the bottom of dishes or flasks using an inverted microscope. Two-dimensional and horizontal observation is insufficient for histological analysis of several cell lines, such as embryonic stem cells or cancer cells, because they form three-dimensional colonies. In the present study, we aimed to establish a more informative method for analysis of such stereoscopic cultured cells. We cultured mouse embryonic stem cells using a temperature-sensitive culture dish, embedded these cells in paraffin, and successfully observed vertical sections of embryonic stem cells. This vertical analysis of the stereoscopic colony emphasized structural features such as the dome shape of naïve pluripotent stem cells. This method could have the potential for analysis of three-dimensional structures and histological preservation in cultured cells.

本文言語英語
ページ(範囲)1479-1481
ページ数3
ジャーナルJournal of Veterinary Medical Science
80
10
DOI
出版ステータス出版済み - 2018

All Science Journal Classification (ASJC) codes

  • 獣医学(全般)

フィンガープリント

「Paraffin-embedded vertical sections of mouse embryonic stem cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル