Partial amino-acid sequence of ECP31, a carrot embryogenic-cell protein, and enhancement of its accumulation by abscisic acid in somatic embryos

Tomohiro Kiyosue, Jiro Nakayama, Shinobu Satoh, Akira Isogai, Akinori Suzuki, Hiroshi Kamada, Hiroshi Harada

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

ECP31, an embryogenic-cell protein from carrot (Daucus carota L.), was purified by sequential column-chromatographic steps and digested by V8 protease on a nitrocellulose membrane. The resultant peptides were separated by reverse-phased column chromatography and sequenced. The sequences obtained were 70-80% homologous to those of a late-embryogenesis-abundant protein (D34) from cotton (Baker et al, 1988, Plant Mol. Biol. 11, 227-291). The level of ECP31 in somatic embryos of carrot was increased by treatment of the embryos with 3.7 · 10-6 M abscisic acid (ABA) for 48 h, and there was no change in this enhanced level for up to 192 h in the presence of ABA. No similar enhancing effect of ABA was observed on the level of ECP31 in embryogenic callus or segments of carrot hypocotyls. In an immunohistochemical analysis, ECP31 was found in epidermal tissue and in the vascular system of ABA-treated somatic embryos.

元の言語英語
ページ(範囲)337-342
ページ数6
ジャーナルPlanta
186
発行部数3
DOI
出版物ステータス出版済み - 2 1 1992

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Abscisic Acid
Daucus carota
somatic embryos
carrots
abscisic acid
Amino Acid Sequence
amino acid sequences
Embryonic Structures
Proteins
proteins
cells
Gossypium
Hypocotyl
plant vascular system
Collodion
Bony Callus
embryo (plant)
hypocotyls
Embryonic Development
Blood Vessels

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

これを引用

Partial amino-acid sequence of ECP31, a carrot embryogenic-cell protein, and enhancement of its accumulation by abscisic acid in somatic embryos. / Kiyosue, Tomohiro; Nakayama, Jiro; Satoh, Shinobu; Isogai, Akira; Suzuki, Akinori; Kamada, Hiroshi; Harada, Hiroshi.

:: Planta, 巻 186, 番号 3, 01.02.1992, p. 337-342.

研究成果: ジャーナルへの寄稿記事

Kiyosue, Tomohiro ; Nakayama, Jiro ; Satoh, Shinobu ; Isogai, Akira ; Suzuki, Akinori ; Kamada, Hiroshi ; Harada, Hiroshi. / Partial amino-acid sequence of ECP31, a carrot embryogenic-cell protein, and enhancement of its accumulation by abscisic acid in somatic embryos. :: Planta. 1992 ; 巻 186, 番号 3. pp. 337-342.
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abstract = "ECP31, an embryogenic-cell protein from carrot (Daucus carota L.), was purified by sequential column-chromatographic steps and digested by V8 protease on a nitrocellulose membrane. The resultant peptides were separated by reverse-phased column chromatography and sequenced. The sequences obtained were 70-80{\%} homologous to those of a late-embryogenesis-abundant protein (D34) from cotton (Baker et al, 1988, Plant Mol. Biol. 11, 227-291). The level of ECP31 in somatic embryos of carrot was increased by treatment of the embryos with 3.7 · 10-6 M abscisic acid (ABA) for 48 h, and there was no change in this enhanced level for up to 192 h in the presence of ABA. No similar enhancing effect of ABA was observed on the level of ECP31 in embryogenic callus or segments of carrot hypocotyls. In an immunohistochemical analysis, ECP31 was found in epidermal tissue and in the vascular system of ABA-treated somatic embryos.",
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AU - Kiyosue, Tomohiro

AU - Nakayama, Jiro

AU - Satoh, Shinobu

AU - Isogai, Akira

AU - Suzuki, Akinori

AU - Kamada, Hiroshi

AU - Harada, Hiroshi

PY - 1992/2/1

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N2 - ECP31, an embryogenic-cell protein from carrot (Daucus carota L.), was purified by sequential column-chromatographic steps and digested by V8 protease on a nitrocellulose membrane. The resultant peptides were separated by reverse-phased column chromatography and sequenced. The sequences obtained were 70-80% homologous to those of a late-embryogenesis-abundant protein (D34) from cotton (Baker et al, 1988, Plant Mol. Biol. 11, 227-291). The level of ECP31 in somatic embryos of carrot was increased by treatment of the embryos with 3.7 · 10-6 M abscisic acid (ABA) for 48 h, and there was no change in this enhanced level for up to 192 h in the presence of ABA. No similar enhancing effect of ABA was observed on the level of ECP31 in embryogenic callus or segments of carrot hypocotyls. In an immunohistochemical analysis, ECP31 was found in epidermal tissue and in the vascular system of ABA-treated somatic embryos.

AB - ECP31, an embryogenic-cell protein from carrot (Daucus carota L.), was purified by sequential column-chromatographic steps and digested by V8 protease on a nitrocellulose membrane. The resultant peptides were separated by reverse-phased column chromatography and sequenced. The sequences obtained were 70-80% homologous to those of a late-embryogenesis-abundant protein (D34) from cotton (Baker et al, 1988, Plant Mol. Biol. 11, 227-291). The level of ECP31 in somatic embryos of carrot was increased by treatment of the embryos with 3.7 · 10-6 M abscisic acid (ABA) for 48 h, and there was no change in this enhanced level for up to 192 h in the presence of ABA. No similar enhancing effect of ABA was observed on the level of ECP31 in embryogenic callus or segments of carrot hypocotyls. In an immunohistochemical analysis, ECP31 was found in epidermal tissue and in the vascular system of ABA-treated somatic embryos.

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