Partial Purification and Characterization of DOPA Quinone Imine Conversion Factor from Larval Hemolymph of the Silkworm, Bombyx mori

Yoichi Aso, Kohji Yamamoto, Takaaki Yoshinaga, Hikaru Yamamoto, Takeshi Yamagami

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

DOPA quinone imine conversion factor was partially purified from larval hemolymph of the silkworm, Bombyx mori, using a novel assay. The factor was labile at 4°C below pH 5 and above pH 11, and at pH 8.5 above 55°C. Activity of the factor was maximal at pH 7.5–9. The factor catalyzed the decolorizations of L-DOPA methyl ester and α-methyl DOPA methyl ester chromes besides DOPA quinone imine. HPLC analysis indicated that the factor-catalyzed decolorization reaction of DOPA quinone imine resulted in the accumulation of dihydroxyindole. Based on spectral changes of α-methyl DOPA methyl ester chrome, it was suggested that the factor catalyzed the intramolecular oxidoreduction of DOPA quinone imine. During the oxidation of L-DOPA with endogenous phenoloxidase, the factor facilitated the formation of melanochrome.

元の言語英語
ページ(範囲)277-281
ページ数5
ジャーナルBioscience, biotechnology, and biochemistry
59
発行部数2
DOI
出版物ステータス出版済み - 1 1 1995

Fingerprint

Imines
Bombyx
Hemolymph
Purification
Esters
Monophenol Monooxygenase
Statistical Factor Analysis
Assays
High Pressure Liquid Chromatography
Oxidation
benzoquinone

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Partial Purification and Characterization of DOPA Quinone Imine Conversion Factor from Larval Hemolymph of the Silkworm, Bombyx mori. / Aso, Yoichi; Yamamoto, Kohji; Yoshinaga, Takaaki; Yamamoto, Hikaru; Yamagami, Takeshi.

:: Bioscience, biotechnology, and biochemistry, 巻 59, 番号 2, 01.01.1995, p. 277-281.

研究成果: ジャーナルへの寄稿記事

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abstract = "DOPA quinone imine conversion factor was partially purified from larval hemolymph of the silkworm, Bombyx mori, using a novel assay. The factor was labile at 4°C below pH 5 and above pH 11, and at pH 8.5 above 55°C. Activity of the factor was maximal at pH 7.5–9. The factor catalyzed the decolorizations of L-DOPA methyl ester and α-methyl DOPA methyl ester chromes besides DOPA quinone imine. HPLC analysis indicated that the factor-catalyzed decolorization reaction of DOPA quinone imine resulted in the accumulation of dihydroxyindole. Based on spectral changes of α-methyl DOPA methyl ester chrome, it was suggested that the factor catalyzed the intramolecular oxidoreduction of DOPA quinone imine. During the oxidation of L-DOPA with endogenous phenoloxidase, the factor facilitated the formation of melanochrome.",
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