Participation of D-serine in the development and reproduction of the silkworm Bombyx mori

Minoru Tanigawa, Chihiro Suzuki, Kimio Niwano, Rensuke Kanekatsu, Hiroyuki Tanaka, Kihachiro Horiike, Kenji Hamase, Yoko Nagata

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

The silkworm Bombyx mori contains high concentrations of free D-serine, an optical isomer of l-serine. To elucidate its function, we first investigated the localization of D-serine in various organs of silkworm larvae, pupae, and adult moths. Using immunohistochemical analysis with an anti-D-serine antibody, we found D-serine in the microvilli of midgut goblet and cylindrical cells and in peripheral matrix components of testicular and ovarian cells. By spectrophotometric analysis, D-serine was also found in the hemolymph and fat body. d-Alanine was not detected in the various organs by immunohistochemistry. Serine racemase, which catalyzes the inter-conversion of l- and D-serine, was found to co-localize with D-serine, and D-serine production from l-serine by intrinsic serine racemase was suggested. O-Phospho-L-serine is an inhibitor of serine racemase, and it was administered to the larvae to reduce the D-serine level. This reagent decreased the midgut caspase-3 level and caused a delay in spermatogenesis and oogenesis. The reagent also decreased mature sperm and egg numbers, suggesting D-serine participation in these processes. D-serine administration induced an increase in pyruvate levels in testis, midgut, and fat body, indicating conversion of D-serine to pyruvate. On the basis of these results, together with our previous investigation of ATP biosynthesis in testis, we consider the possible involvement of D-serine in ATP synthesis for metamorphosis and reproduction.

元の言語英語
ページ(範囲)20-29
ページ数10
ジャーナルJournal of insect physiology
87
DOI
出版物ステータス出版済み - 4 1 2016

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Bombyx
Bombyx mori
silkworms
serine
Serine
Reproduction
midgut
Fat Body
Pyruvic Acid
fat body
Larva
Testis
Adenosine Triphosphate
testes
Oogenesis
Pupa
Sperm Count
Goblet Cells
Hemolymph
Moths

All Science Journal Classification (ASJC) codes

  • Physiology
  • Insect Science

これを引用

Tanigawa, M., Suzuki, C., Niwano, K., Kanekatsu, R., Tanaka, H., Horiike, K., ... Nagata, Y. (2016). Participation of D-serine in the development and reproduction of the silkworm Bombyx mori. Journal of insect physiology, 87, 20-29. https://doi.org/10.1016/j.jinsphys.2016.01.006

Participation of D-serine in the development and reproduction of the silkworm Bombyx mori. / Tanigawa, Minoru; Suzuki, Chihiro; Niwano, Kimio; Kanekatsu, Rensuke; Tanaka, Hiroyuki; Horiike, Kihachiro; Hamase, Kenji; Nagata, Yoko.

:: Journal of insect physiology, 巻 87, 01.04.2016, p. 20-29.

研究成果: ジャーナルへの寄稿記事

Tanigawa, Minoru ; Suzuki, Chihiro ; Niwano, Kimio ; Kanekatsu, Rensuke ; Tanaka, Hiroyuki ; Horiike, Kihachiro ; Hamase, Kenji ; Nagata, Yoko. / Participation of D-serine in the development and reproduction of the silkworm Bombyx mori. :: Journal of insect physiology. 2016 ; 巻 87. pp. 20-29.
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