Paternal allele influences high fat diet-induced obesity

Sumiyo Morita, Takuro Horii, Mika Kimura, Yuji Arai, Yasutomi Kamei, Yoshihiro Ogawa, Izuho Hatada

研究成果: ジャーナルへの寄稿学術誌査読

20 被引用数 (Scopus)

抄録

C57BL/6J (B6) mice are susceptible to high-fat diet (HFD)-induced obesity and have been used in metabolism research for many decades. However, the genetic component of HFD-induced obesity has not yet been elucidated. This study reports evidence for a paternal transmission of HFD-induced obesity and a correlated expression of Igf2 and Peg3 (paternal expressed gene 3) imprinted genes. We found that PWK mice are resistant to HFD-induced obesity compared to C57BL/6J mice. Therefore, we generated and analyzed reciprocal crosses between these mice, namely; (PWKxB6) F1 progeny with B6 father and (B6xPWK) F1 progeny with PWK father. The (PWKxB6) F1 mice were more sensitive to diet-induced obesity compared to (B6xPWK) F1 mice, suggesting a paternal transmission of diet-induced obesity. Expression analysis of imprinted genes in adipocytes revealed that HFD influences the expression of some of the imprinted genes in adipose tissue in B6 and PWK mice. Interestingly, Igf2 and Peg3, which are paternally expressed imprinted genes involved in the regulation of body fat accumulation, were down-regulated in B6 and (PWKxB6) F1 mice, which are susceptible to HFD-induced obesity, but not in PWK and (B6xPWK) F1 mice, which are resistant. Furthermore, in vitro analysis showed that Igf2, but not Peg3, had an anti-inflammatory effect on TNF-α induced MCP-1 expression in adipocytes. Taken together, our findings suggest that the down-regulation of Igf2 and Peg3 imprinted genes in adipocytes may be involved in the paternal transmission of HFD-induced obesity.

本文言語英語
論文番号e85477
ジャーナルPloS one
9
1
DOI
出版ステータス出版済み - 1月 8 2014
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

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