Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori

研究成果: ジャーナルへの寄稿記事

28 引用 (Scopus)

抄録

To investigate the pathogenicity of Pseudomonas aeruginosa in insects, a gacA mutant of P. aeruginosa PA01 was constructed by site-directed mutagenesis. The mutant was designated as C1. C1 was less virulent to Bombyx mori than the parent strain. To complement the gacA gene, P. aeruginosa C1 was transformed with the broad host range plasmid pJB3Km1 carrying a 3.9-kbp gacA fragment. The expression of the gacA mRNA in C1 (pgacA) was detected. In addition, the complemented mutant restored the level and timing of pyocyanin production, indicating that functional GacA is produced in the complemented strain. However, no significant difference was observed between C1 and C1 (pgacA) with respect to the killing of B. mori larvae.

元の言語英語
ページ(範囲)181-186
ページ数6
ジャーナルFEMS microbiology letters
244
発行部数1
DOI
出版物ステータス出版済み - 3 1 2005

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Bombyx
Pseudomonas aeruginosa
Virulence
Pyocyanine
Host Specificity
Site-Directed Mutagenesis
Larva
Insects
Plasmids
Messenger RNA
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

これを引用

Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori. / Chieda, Yuuka; Iiyama, Kazuhiro; Yasunaga-Aoki, Chisa; Lee, Man; Kusakabe, Takahiro; Shimizu, Susumu.

:: FEMS microbiology letters, 巻 244, 番号 1, 01.03.2005, p. 181-186.

研究成果: ジャーナルへの寄稿記事

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abstract = "To investigate the pathogenicity of Pseudomonas aeruginosa in insects, a gacA mutant of P. aeruginosa PA01 was constructed by site-directed mutagenesis. The mutant was designated as C1. C1 was less virulent to Bombyx mori than the parent strain. To complement the gacA gene, P. aeruginosa C1 was transformed with the broad host range plasmid pJB3Km1 carrying a 3.9-kbp gacA fragment. The expression of the gacA mRNA in C1 (pgacA) was detected. In addition, the complemented mutant restored the level and timing of pyocyanin production, indicating that functional GacA is produced in the complemented strain. However, no significant difference was observed between C1 and C1 (pgacA) with respect to the killing of B. mori larvae.",
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AU - Kusakabe, Takahiro

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