Pattern of c-Fos expression induced by tail suspension test in the mouse brain

Kentaro Hiraoka, Keisuke Motomura, Satoru Yanagida, Ayako Ohashi, Nozomi Ishisaka-Furuno, Shigenobu Kanba

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

The tail suspension test (TST) has been widely used as a screening assay for antidepressant drugs. However, the neural substrates underlying the stress response and antidepressant-like effect during the TST remain largely unknown despite the prevalence of this test. In the present study, we used immunohistochemistry to examine alterations in c-Fos expression as a measure of neuronal activity in the mouse brain after acute administration of the antidepressant drugs nortriptyline or escitalopram (or saline as a control) with or without a subsequent TST session. We found that without the TST session, nortriptyline administration enhanced the density of c-Fos-immunoreactive cells in regions of the central extended amygdala, paraventricular hypothalamic nucleus, and relevant regions of the brain stem, whereas escitalopram did not change c-Fos expression in any region. Following the TST in the absence of antidepressant drugs, we observed a significant increase in c-Fos-positive cell density in a number of brain regions within the limbic telencephalon, hypothalamus, and brain stem. We detected a statistically significant interaction using an analysis of variance between the main effects of the drug and stress response in four regions: the infralimbic cortex, lateral septal nucleus (intermediate part), ventrolateral preoptic nucleus, and solitary nucleus. Following the TST, escitalopram but not nortriptyline increased c-Fos-positive cell density in the infralimbic cortex and ventrolateral preoptic nucleus, whereas nortriptyline but not escitalopram increased c-Fos expression in the solitary nucleus. Both antidepressants significantly increased c-Fos expression in the lateral septal nucleus (intermediate part). The present results indicate that neuronal activity increases in septo-hypothalamic regions and related structures, especially the lateral septal nucleus, following administration of drugs producing an antidepressant-like effect in mice subjected to the TST.

元の言語英語
記事番号e00316
ジャーナルHeliyon
3
発行部数6
DOI
出版物ステータス出版済み - 6 1 2017

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Hindlimb Suspension
Antidepressive Agents
Nortriptyline
Citalopram
Brain
Septal Nuclei
Solitary Nucleus
Preoptic Area
Brain Stem
Cell Count
Telencephalon
Paraventricular Hypothalamic Nucleus
Hypothalamus
Analysis of Variance
Immunohistochemistry
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • General

これを引用

Hiraoka, K., Motomura, K., Yanagida, S., Ohashi, A., Ishisaka-Furuno, N., & Kanba, S. (2017). Pattern of c-Fos expression induced by tail suspension test in the mouse brain. Heliyon, 3(6), [e00316]. https://doi.org/10.1016/j.heliyon.2017.e00316

Pattern of c-Fos expression induced by tail suspension test in the mouse brain. / Hiraoka, Kentaro; Motomura, Keisuke; Yanagida, Satoru; Ohashi, Ayako; Ishisaka-Furuno, Nozomi; Kanba, Shigenobu.

:: Heliyon, 巻 3, 番号 6, e00316, 01.06.2017.

研究成果: ジャーナルへの寄稿記事

Hiraoka, K, Motomura, K, Yanagida, S, Ohashi, A, Ishisaka-Furuno, N & Kanba, S 2017, 'Pattern of c-Fos expression induced by tail suspension test in the mouse brain', Heliyon, 巻. 3, 番号 6, e00316. https://doi.org/10.1016/j.heliyon.2017.e00316
Hiraoka, Kentaro ; Motomura, Keisuke ; Yanagida, Satoru ; Ohashi, Ayako ; Ishisaka-Furuno, Nozomi ; Kanba, Shigenobu. / Pattern of c-Fos expression induced by tail suspension test in the mouse brain. :: Heliyon. 2017 ; 巻 3, 番号 6.
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abstract = "The tail suspension test (TST) has been widely used as a screening assay for antidepressant drugs. However, the neural substrates underlying the stress response and antidepressant-like effect during the TST remain largely unknown despite the prevalence of this test. In the present study, we used immunohistochemistry to examine alterations in c-Fos expression as a measure of neuronal activity in the mouse brain after acute administration of the antidepressant drugs nortriptyline or escitalopram (or saline as a control) with or without a subsequent TST session. We found that without the TST session, nortriptyline administration enhanced the density of c-Fos-immunoreactive cells in regions of the central extended amygdala, paraventricular hypothalamic nucleus, and relevant regions of the brain stem, whereas escitalopram did not change c-Fos expression in any region. Following the TST in the absence of antidepressant drugs, we observed a significant increase in c-Fos-positive cell density in a number of brain regions within the limbic telencephalon, hypothalamus, and brain stem. We detected a statistically significant interaction using an analysis of variance between the main effects of the drug and stress response in four regions: the infralimbic cortex, lateral septal nucleus (intermediate part), ventrolateral preoptic nucleus, and solitary nucleus. Following the TST, escitalopram but not nortriptyline increased c-Fos-positive cell density in the infralimbic cortex and ventrolateral preoptic nucleus, whereas nortriptyline but not escitalopram increased c-Fos expression in the solitary nucleus. Both antidepressants significantly increased c-Fos expression in the lateral septal nucleus (intermediate part). The present results indicate that neuronal activity increases in septo-hypothalamic regions and related structures, especially the lateral septal nucleus, following administration of drugs producing an antidepressant-like effect in mice subjected to the TST.",
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AB - The tail suspension test (TST) has been widely used as a screening assay for antidepressant drugs. However, the neural substrates underlying the stress response and antidepressant-like effect during the TST remain largely unknown despite the prevalence of this test. In the present study, we used immunohistochemistry to examine alterations in c-Fos expression as a measure of neuronal activity in the mouse brain after acute administration of the antidepressant drugs nortriptyline or escitalopram (or saline as a control) with or without a subsequent TST session. We found that without the TST session, nortriptyline administration enhanced the density of c-Fos-immunoreactive cells in regions of the central extended amygdala, paraventricular hypothalamic nucleus, and relevant regions of the brain stem, whereas escitalopram did not change c-Fos expression in any region. Following the TST in the absence of antidepressant drugs, we observed a significant increase in c-Fos-positive cell density in a number of brain regions within the limbic telencephalon, hypothalamus, and brain stem. We detected a statistically significant interaction using an analysis of variance between the main effects of the drug and stress response in four regions: the infralimbic cortex, lateral septal nucleus (intermediate part), ventrolateral preoptic nucleus, and solitary nucleus. Following the TST, escitalopram but not nortriptyline increased c-Fos-positive cell density in the infralimbic cortex and ventrolateral preoptic nucleus, whereas nortriptyline but not escitalopram increased c-Fos expression in the solitary nucleus. Both antidepressants significantly increased c-Fos expression in the lateral septal nucleus (intermediate part). The present results indicate that neuronal activity increases in septo-hypothalamic regions and related structures, especially the lateral septal nucleus, following administration of drugs producing an antidepressant-like effect in mice subjected to the TST.

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