Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts

Kenji Okuda, Anne Laure Chateigner-Boutin, Takahiro Nakamura, Etienne Delannoy, Mamoru Sugita, Fumiyoshi Myouga, Reiko Motohashi, Kazuo Shinozaki, Ian Small, Toshiharu Shikanai

研究成果: ジャーナルへの寄稿学術誌査読

185 被引用数 (Scopus)

抄録

The plant-specific DYW subclass of pentatricopeptide repeat proteins has been postulated to be involved in RNA editing of organelle transcripts. We discovered that the DYW proteins CHLORORESPIRATORY REDUCTION22 (CRR22) and CRR28 are required for editing of multiple plastid transcripts but that their DYW motifs are dispensable for editing activity in vivo. Replacement of the DYW motifs of CRR22 and CRR28 by that of CRR2, which has been shown to be capable of endonucleolytic cleavage, blocks the editing activity of both proteins. In return, the DYW motifs of neither CRR22 nor CRR28 can functionally replace that of CRR2. We propose that different DYW family members have acquired distinct functions in the divergent processes of RNA maturation, including RNA cleavage and RNA editing.

本文言語英語
ページ(範囲)146-156
ページ数11
ジャーナルPlant Cell
21
1
DOI
出版ステータス出版済み - 1月 2009

!!!All Science Journal Classification (ASJC) codes

  • 植物科学
  • 細胞生物学

フィンガープリント

「Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル