Peptide array for the comprehensive analysis of intracellular signals aiming to drug screening

Yoshiki Katayama, Tatsuhiko Sonoda, Yusuke Inoue, Takayuki Yamaji, Aishan Han, Kazuki Inamori, Osamu Okitsu, Masaharu Murata, Takuro Niidome

研究成果: Contribution to conferencePaper査読

抄録

New peptide array, on which many substrate peptides for protein kinases are immobilized, was developed. If the array was treated with isolated protein kinases or cell lysate, each peptide was phosphorylated dependent on their properties, after the optimized immobilization and masking procedures. Phosphorylation in each spot was detected by fluorescence on glass slide or 2 dimensional SPR on gold chip using anti-phospho antibody. In the glass array using Cy-3 labeled antibody for the detection, drug-specific profiles of peptide phosphorylation were obtained. Using this method, new assay system for the drug screening or fuctional analysis of unknown gene may be possible.

本文言語英語
ページ4849-4850
ページ数2
出版ステータス出版済み - 2005
イベント54th SPSJ Symposium on Macromolecules - Yamagata, 日本
継続期間: 9 20 20059 22 2005

その他

その他54th SPSJ Symposium on Macromolecules
国/地域日本
CityYamagata
Period9/20/059/22/05

All Science Journal Classification (ASJC) codes

  • 工学(全般)

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