Peptide imprinted polymer nanoparticles: A plastic antibody

Yu Hoshino, Takashi Kodama, Yoshio Okahata, Kenneth J. Shea

研究成果: Contribution to journalArticle査読

303 被引用数 (Scopus)

抄録

A novel method for preparation of biomacromolecular imprinted nanoparticles is described. Combinations of functional monomers were polymerized in the presence of the imprinting peptide melittin in aqueous solution at room temperature to produce a small library of polymer nanoparticles. The template peptide and unreacted monomers are subsequently removed by dialysis. Nanoparticles (NPs) from the library were evaluated for their binding to melittin by 27 MHz QCM analysis. NPs prepared with optimized functional monomer combinations bind strongly to the target molecule. Nanoparticles that were polymerized in the absence of template peptide were found to have little affinity to the peptide. Binding affinity and the size of imprinted particles are comparable to those of natural antibodies. They interact specifically with the target peptide and show little affinity for other proteins. These NPs are of interest as inert and stable substitutes for antibodies. Extension of this approach to other targets of biological importance and the applications of these materials are currently being evaluated.

本文言語英語
ページ(範囲)15242-15243
ページ数2
ジャーナルJournal of the American Chemical Society
130
46
DOI
出版ステータス出版済み - 11 19 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

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