Peptide substrates for Rho-Associated kinase 2 (Rho-Kinase 2/ROCK2)

Jeong Hun Kang, Daisuke Asai, Akira Tsuchiya, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Peptide substrates sensitive for a certain protein kinase could be important for new-drug development and to understand the mechanism of diseases. Rho-associated kinase (Rho-kinase/ROCK) is a serine/threonine kinase, and plays an important part in cardiovascular disease, migration and invasion of tumor cells, and in neurological disorders. The purpose of this study was to find substrates with high affinity and sensitivity for ROCK2. We synthesized 136 peptide substrates from protein substrates for ROCK2 with different lengths and charged peptides. Incorporation of 32P [counts per minute (CPM)] for each peptide substrate was determined by the radiolabel assay using [γ- 32P]ATP. When the top five peptide substrates showing high CPMs (R4, R22, R133, R134, and R135) were phosphorylated by other enzymes (PKA, PKCα, and ERK1), R22, R133, and R135 displayed the highest CPM level for ROCK2 compared with other enzymes, whereas R4 and R134 showed similar CPM levels for ROCK2 and PKCα. We hypothesize that R22, R133, and R135 can be useful peptide substrates for ROCK2.

元の言語英語
記事番号e22699
ジャーナルPloS one
6
発行部数7
DOI
出版物ステータス出版済み - 8 1 2011

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rho-Associated Kinases
phosphotransferases (kinases)
peptides
Peptides
Substrates
nervous system diseases
new drugs
Protein-Serine-Threonine Kinases
Enzymes
enzymes
Nervous System Diseases
threonine
protein kinases
Protein Kinases
serine
cardiovascular diseases
Cardiovascular Diseases
Adenosine Triphosphate
Tumors
Assays

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

これを引用

Peptide substrates for Rho-Associated kinase 2 (Rho-Kinase 2/ROCK2). / Kang, Jeong Hun; Asai, Daisuke; Tsuchiya, Akira; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki.

:: PloS one, 巻 6, 番号 7, e22699, 01.08.2011.

研究成果: ジャーナルへの寄稿記事

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AU - Asai, Daisuke

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AU - Mori, Takeshi

AU - Niidome, Takuro

AU - Katayama, Yoshiki

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