Peptides binding to a Gb3 mimic selected from a phage library

Yoshiko Miura, Yuuki Sasao, Masamichi Kamihira, Akio Sakaki, Shinji Iijima, Kazukiyo Kobayashi

研究成果: Contribution to journalArticle査読

23 被引用数 (Scopus)

抄録

Peptides binding to a Gb3 mimic were selected from 12-mer peptide library. The self-assembled monolayer (SAM) of a Gb3 mimic was formed on the gold surface, and biopanning was carried out with the phage display peptide library. After three rounds of biopanning, four individual sequences were obtained from 10 phage clones, and the selected peptides having the specific 7-mer sequence (FHENWPS) showed affinities to the Gb3 mimic as strong as to RCA120. Molecular dynamics calculations suggested that the peptides bound to the Gb3 mimic by hydrophobic interaction and hydrogen bonding formation, and the cooperative interactions played an important role in the recognition. The Stx-1 binding was inhibited by the peptides.

本文言語英語
ページ(範囲)131-138
ページ数8
ジャーナルBiochimica et Biophysica Acta - General Subjects
1673
3
DOI
出版ステータス出版済み - 8 4 2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学

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